欢迎来到天天文库
浏览记录
ID:32005542
大小:8.94 MB
页数:65页
时间:2019-01-30
《山苍子无性繁殖技术的.研究》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、福建农转大学2008届硕士学位论文Usingcompletelyrandomizedexperimentaldesign,SanmingprovenanceoftheShootbrancheshavethehi曲estlaunchrate(81.02%)andProliferationrate(3.06).Sanmingprovenancewastheoptimal,JiangleprovenanceWasthesecondoneandShaxianprovenanceoftheworstlrain.differentbranchespartsoftheexplants
2、havethedifferentlaunchrateandtheproliferationrate,theshoothavethehighestlaunchrateandtheproliferation,andthenfromtoptobottom,thelaunchrateandtheproliferationratehasdecreased.1.5Effectofcontaminationrate、launchrateandproliferationondifferentmonthsofLitseacubebaCollectedindifferentmonths,t
3、hepollutionrateandtherateofproliferationactivateddifferent,SeptemberandNovemberWaSabeRertimeinthesecondhalfofthetrainingtime,March,AprilSabettertimeinthefirsthalfofthetrainingtime,withthehigherlaunchrateandproliferation.1.6ThesubcultureofLitseacubebaUsingoahogonaldesign,andselectedthefir
4、stsubculturemediumforthebestWasMS+I.0mg/LBA+0.5mg/LKT+0.4mg/LIBA+309/LSucrose.thesecondsubculturemediumWasMS+I.Omg/LBA+0.1mg/LKT+0.4mg/LIBA+309/LSucrose+O.59/LAC.thethirdsubculturemediumwasMS+0.5mg/LBA+0.2mg/LIBA+209/LSucrose+o.59eLAC.1.7TherootingcultureandtransplantingofLitseacubebaUsi
5、ngorthogonaldesign,,takingintotheeffectofdifferenthormonesontherootingofLitseaCubeba,selectedthebestmediumoftherootingculturewasI/2MS+0.Smg/LIBA+0.2mgCLIAA+209/LSucrose.TransplantingmediaWasthesandinthefirst,andthentransplanttothemedia(sand:yellowsoil:peatsoil=1:1:1)later.2.Callusinducti
6、on,subcultureandregenerationofLitseacubebaUsingthreedifferentexplants(stems,leaves,petioles)toinducethecallus,andestablishedcallussystemofLi括eaCubeba.Differentexplants(stems,leaves,petioles)haddifferentdifficultytoinducethecallus:stems>petioles>leaves.stemswerethebestmaterialofa11.Theopt
7、imummedisofstemcallusinductionwasMS+2.Omg/t,2,4-D+1.Omg/LBA+I.0mg/LKT+309/LSucrose.TheoptimummedisofleavecallusinductionWasMS+I.5mg/L2,4-D+I.0mg/LBA+0.5mg/LKT+309/LSucrose.TheoptimummedisofpetiolescallusinductionwasMS+I。5mg/L2,4-D+1。OmgCt,BA+I.0mg/LKT+309/LSucrose.Thebest
此文档下载收益归作者所有