金钱草总黄酮的提取、纯化与分析

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1、ToextractChristinaLoosestrifeHerbtotalflavonoids，anorthogonaltablehasbeenusedtofindthebestprocedureforMAEofflavonoids．Theoptimunconditionforprocedureisextracting30minat60。Cwith8foldsof50％ethan01．Theethanolconcentrationwassignificantamongallfactorsonthetest，extractiontemp

2、eraturewasinthenextplace，whileextractiontimeandfoldswereunconspicuous．TheflavonoidscontentsinChristinaLoosestrifeHerbis5．58％byMAE，80％morethantraditionaldistillationextractionmethod，whichonlvmade3．09％yield．Comparingwithtraditionalextraction，TheadvamagesofMAEineveryimporta

3、ntfactorsofyield，timeandconsumptionofsolventet．Afterextraction，theChristinaLoosestrifeHerbtotalflavonoidshavebeenpurifiedbymacroporousresins．TheD101，AB-8andNKA-9macroporousadsorbentswereinvestigatedtoseparateandpurifyChristinaLoosestrifeHerbtotalflavonoids，evaluatingbyad

4、sorptioncapacity,elutionratioandproductpurity．Amongthethreetypesofmacroporousadsorbents，theNKA_9typeshowsbetterpropertywiththeadsorptioncapacityof51．2mg·mL。1andtheelutionratioof96．5％．Accordingtotheresultofexperiment．Thebestmethodconditionis：extractsampleconcentrationof20

5、mg‘mL～，6timeswashingwaterandelutionreagentof50％ThepurityofChristinaLoosestrifeHerbtotalflavonoidsproductcanreach60．8％afterseparationandpurification．Theadsorbentcanbeused4timesrepeatedly．Moreover,tOestablishakineticmodelforthisseparationprocess，thecurvesofdynamicadsorptio

6、n-elutionprocesshavebeenpitted，andthekineticequationhasbeenpresentasy=u(1一e也x)．ContentmensurateofQuercetinandKaempferolinChristinaLoosestrifeHerbtotalflavonoidsbyHPLCafterseparationandpurification．Theoptimumprocedureis：separationofthesecomponentswasachievedonaC19Nova--pa

7、kcolumnwithgradientelutionusingmethanol--water--phosphateacid(500：500：1)asmobilephase，constantflowrateof1．0mL‘min～，columntemperatuewas25。CandUVdetectionwassetat360nm．RetentiontimeofQuercetinisapproximately5．1minand8．8minofKaempfer01．ThetwocalibrationCurvesshowsgoodlinear

8、ity(r2>0．999)withtestranges．Intra-assayprecisionofbothcomponentsare0．31％and0．27％，theaveragerecoveryrate