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1、CHITOSANONANDRAEANUMTISSUEINHIBITIONOFBACTERIACONTAMINATIONAbstract:Thefungiwasisolatedandpurifiedfrompollutedanthuriumsubculturemedium,identifiedtobeDeuteromycotinaHyphpmycetesHyphomycetalesDematiaceaeandtrichoderma.Theinhibitioneffectofthethreekindsofchit
2、osanonthefungiwasconducted.Theresultsshowedthatthechitosanhadinhibitioneffectonthefungi.Thehigherconcentrationsofchitosanhadabetteinhibitioneffectthanthelowerones.Theinhibitioneffectof50000and250000molecularweightchitosanisbetterthanthatofthe3000molecularwe
3、ight.Itispreliminarilyprovedthatchitosanhadgoodinhibitioneffectonthefungifrompollutedtissueculture,andwillhavegoodapplicationvalueinthefieldsofpollutionpreventioninflowerstissueculture.Keywords:tissueculture；contamination；Chitosan；inhibitioneffectPlanttissu
4、eculture,notonlyinplantbreeding,rapidpropagationofseedlingsandtheproductionofusefulsecondarymetabolites,suchastheuseofawiderangeofplantgeneticengineeringisandPlantMolecularBiology,oneoftheimportantfoundationforthestudy[1].Pollution,browning,glassandtissuecu
5、lturearethemainproblems,includingpollutionofthemostcommon[2].Thereforetakeeffectivepreventionandcontrolmeasurestoreducethechanceofcontaminationoccurred,tissuecultureisanimportantguaranteeforsuccess.Chitosan(chitosan)isthechitininstrongalkalineconditionsfrom
6、B.AftertheformationofacylanimportantderivativeisdeterminedbyanumberofN-acetylglucosaminethroughβ-(1-4)glycosidicbondlinkingthenatureof18itmorelively.Hasnaturalantibacterialpropertiesofchitosan,andabroadspectrumantimicrobial,inrecentyears,chitosaninvariousfi
7、eldssuchasagriculture,medicine[3],manufacture[4],food[5],etc.Theuseofgreatimportance.Tissuecultureofthechitosanintheapplicationofinhibitionhasnotbeenreported.Thisexperimenttobefromthecontaminatedmediumsubcultureandraeanumextraction,purificationandidentifica
8、tionofbacteriacontamination,withdifferentmolecularweightchitosananddifferentconcentrationsoftheirinhibition,andexplorethebestinhibitoryeffectofchitosanmolecularweightandthebestinhibitoryconcentration,i
