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《大鼠白介素β (il-β)酶联免疫分析试剂盒使用说明书（elisa kit for rat interleukin beta (il- beta)）》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、大鼠白介素1β(il-1β)酶联免疫分析试剂盒使用说明书（ELISAkitforratinterleukin1beta(IL-1beta)）ELISAkitforratinterleukin1beta(IL-1beta)ThiskitisforresearchuseonlyTestrange:3.12pg/ml-200pg/mlMinimumdetectionlimit:0.78pg/mlSpecificity:thiskitallowssimultaneousdetectionofnaturalorrecombinantratIL-1betaand
2、hasnocrossreactivitywithotherrelatedproteins.Validity:6monthsProspectiveapplication:ELISAmethodforquantitativedeterminationofIL-1,betainserum,plasma,cellculturesupernatantorotherrelatedbiologicalfluidsinrats.Explain1.kitkept:-20degrees(whennotlonger);2-8degrees(frequentlyused).
3、2.,concentratedliquidwashatlowtemperaturewillbesaltedout,dilutedinthewaterbathheating,helpdissolve.3.,theEnglishandChinesemanualmaybeoutoforder.PleaseusetheEnglishmanual.4.thenewlyopenedenzymeplateholemaycontainasmallamountofwatersamples,thisisnormal,andwillnothaveanyeffectonth
4、eresultsoftheexperiment.SummaryInterleukin-1(IL-1),alsoknownaslymphocytestimulatingfactor,isdividedintotwokinds:IL-1alphaandIL-1beta.Theyareexpressedbydifferentgenes,themolecularweightis15kDa,butthePIis5and7respectively.Bothwerecomposedof159and153aminoacids,withonly26%homologyb
5、etweenaminoacidsequences,butwerecombinedwiththesamemembranereceptor.IL-1isproducedmainlybymonocytesandmacrophagesintheblood.Tlymphocytes,Blymphocytes,variousepithelialcells,endothelialcellsandstromalcellsalsoproduceIL-1.IL-1inthebloodismainlyIL-1betaproducedbymonocytesandmacrop
6、hages.IL-1isamajorregulatorofacuteimmuneresponseandplaysafundamentalroleintheactivationofTcellsandtheinductionofIL-2production.Theseregulatoryactionsareproducedmainlybytheactionofthebonemarrowstemcellsinthenervoussystem.MostoftheseregulatoryeffectsaredirectlyproducedbyIL-1beta,
7、butsomeoftheseeffectsaresynergisticwithothercytokinessuchasIL-6,IFN,andTNF.NormalpeopledonotcontainIL-1,beta,oraverylowlevelofblood.ElevatedlevelsofIL-1indicatetissuedamageorinfectioninthebody,suchasstageenteritis,septicemia,etc..Inflammationanddamagetothenervoussystemresultsin
8、elevatedlevelsofIL-1inthecerebrospinalfluid.Elevatedle
