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1、口服当归补血汤后阿魏酸在大鼠体内的组织分布论文王永禄,王丽瑶，陈国广，张东旭，韦萍【摘要】目的研究大鼠灌胃给予当归补血汤后阿魏酸在体内的组织分布特性。方法采用高效液相色谱法；色谱柱为KromasilC18（4.6mm×150mm,5μm）；流动相为0.2%甲醇乙腈-0.2%磷酸0.2%三乙胺水溶液（18：82）；检测波长322nm；柱温40℃；流速0.8ml/min。结果各组织中的阿魏酸在10～400ng范围内与峰面积呈良好的线性关系；回收率平均为87.97%±1.95%；日内和日间的RSD分别1.92%～3.87
2、%和3.21%～6.39%之间。阿魏酸在各组织器官中浓度差异较大。结论大鼠灌胃给予当归补血汤后阿魏酸主要在肝脏和肾脏分布，阿魏酸不能通过血脑屏障。【关键词】当归补血汤；阿魏酸；反相-高效液相色谱；组织分布；大鼠Abstract:ObjectiveToinvestigatethetissuedistributionofferulicacidinratsafteroraladministrationofTangkueiblood-supplementingdecoction.MethodsHPLCn:KromasilC
3、18（150mm×4.6mm，5μm）;mobilephase:0.2%methanolacetonitrile-0.2%phosphoricacid0.2%triethylaminesolution（18：82）;detection;temperaturel/min.ResultsThelinearrangesofferulicacidintheheart,liver,spleen,lungandkidneyajordistributionorgansofferulicacidinratsareliverandk
4、idney.Ferulicacidcannotpassthoughtheblood-barrier.Keyentingdecoction;Ferulicacid;Tissuedistribution；RatsTangkueiBlood-SupplementingDecoctionhasbeenia,femaleirregularmenstruationandamenorrhoeainthetraditionalChinesemedicineforhundredsofyears.Previousstudieshave
5、demonstratedthatTangkueiBlood-SupplementingDecoctioncaninhibitlipidperoxidation,maintainactivitiesofSODandGSH,enhancecellabilityagainstoxidation,improveimmunefunction,andlessenischemia/reperfusioninjuryintheheartandthebrain［1］.Ferulicacid,oneoftheimportantpone
6、ntsofTangkueiBlood-SupplementingDecoction［2］,.freelinishedtheproductionofH2O2inhepatocytesandprotectedmembranelipidafterischemia/reperfusion.Italsoplaysaroleofdilatingbloodvessel［3］,inhibitingplateletaggregation,inhibitinginflammatoryreaction,anti-oxydationand
7、cleaningfreeradical,actingoneliminatingandinhibiting.Inthepresentstudy,thetissuedistributionofferulicacidinratsanceliquidchromatographyafteroraladministrationofTangkueiblood-supplementingdecoction.1Materialandmethods1.1ApparatusandreagentsTheDIONEXHPLCsystemco
8、nsistedofaP680quaternarypumpandaUVD170UvariableatographicostatedcolumnpartmentHeng-aoCo.(Tianjin,China).Ferulicacid(FA)aceuticalandBiologicalProducts(batchnumber:0773-9910;foruseof
