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1、%264%ChineseJournalofCoalIndustryMedicineFeb.2010,Vol.13,No.2文章编号1007-9564(2010)02-0264-03肝素化羊脱细胞真皮基质对大鼠深度创面愈合的影响*016000内蒙古乌海市人民医院烧伤科王志刚王凌峰关键词肝素;羊皮;脱细胞真皮基质;烧伤,深度;大鼠摘要目的制备肝素化羊脱细胞真皮基质(ADM),观察其对大鼠深度烫伤创面愈合的影响。方法制成异种(羊)ADM,再用低分子肝钠素注射液浸泡处理,合成肝素化羊ADM。对肝素化羊ADM进行组织学观察,测定吸附量,进行细菌、真菌培养。
2、!96只Wistar大鼠制作背部深度烫伤模型,随机分为4组,A组(n=24)肝素化羊ADM覆盖创面,B组(n=24)异种(羊)ADM覆盖创面,C组(n=24)肝素钠乳膏涂沫创面,D组(n=24)为对照组。测算3,7,14,21d创面愈合率,于7,14d光镜观察创面组织学变化,于14d电镜观察成纤维细胞结构。观察各组创面、粪、尿有无出血现象。结果肝素化羊ADM质感柔软有弹性,不易断裂。含有低分子肝素钠(4047.50∀497.49)IU/g。光镜检查、电镜检查未见表皮和真皮细胞残留,胶原结构基本完整。细菌和真菌培养呈阴性。!伤后14,21d,A组创面愈合率优于B,C,
3、D组(P<0.05)。伤后14d光镜观察,A组与B,C,D组比较表皮组织、肉芽组织、胶原纤维明显增多。伤后14d电镜观察,A组与B,C,D组比较成纤维细胞细胞器更丰富。各组动物创面、粪便及尿液均无出血现象。结论肝素化羊ADM能够促进大鼠深度创面愈合,适合用作生物敷料。EFFECTSOFHEPARINIZEDSHEEPACELLULARDERMISMATRIXONWOUNDHEALINGOFDEEPPARTIAL-THICKNESSBURNINRATSWangZhigang,WangLingfeng.DepartmentofBurns,ThePeople'shosp
4、italofWuhaiCity,Wuhai016000,ChinaKeywordsheparin;sheepskin;acellulardermalmatrix;deeppartialthicknessburn;ratAbstractObjectiveTopreparetheheparinizedsheepacellulardermalmatrixandinvestigateitseffectsonwoundhealingofdeeppartial-thicknessburninrats.MethodsSheepacellulardermalmatrixwa
5、sfabricatedandimmersedinheparinsodiuminjectiontopreparetheheparinizedsheepacellulardermal.Thehistologicalcharacteristicsweretestedwithopticalmicroscopeandelectronmicroscope.Bacteriaandfungalcultureweretested.Theheparincontentwasalsocalculated.!96Wistarratsweresubjectedtodeeppartialthickn
6、essscaldingandwererandomlydividedintoAgroup(n=24,withheparinizedsheepskinacellulardermalmatrixonthewounds),Bgroup(n=24,withsheepacellulardermalmatrixonthewounds),Cgroup(n=24,withheparinsodiumcreamonthewounds),andDgroup(n=24,withgauzeonthewoundsascontrol).Thehealingratesofthetraumaticwoun
7、dswerecomparedat3,7,14and21postburndays(PBD).Thegrowthofepidermis,granulationtissueandcollagenfiberswereevaluatedunderlightmicroscopeon7and14PBD.Thegrowthoffibroblastwasobservedundertransmissionelectronmicroscopeon14PBD.Tofindoutwhetherbleedingsideeffectswereoccurring,th
