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1、中国水稻科学(ChinJRiceSci),２０１５,２９(４):４２４－４３０http://www．ricesci．cn４２４DOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０４．０１２灰飞虱LHSC７０基因表达特性及功能王利华∗单丹方继朝∗(江苏省农业科学院植物保护研究所,南京２１００１４;∗通讯联系人,EＧmail:wlhyang＠sohu．com,fangjc＠１２６．com)ExpressionProfilingandFunctionAnalysesofLHSC７０inLaodelphaxstriatellusWAN
2、GLiＧhua∗,SHANDan,FANGJiＧchao∗(InstituteofPlantProtection,JiangsuAcademyofAgriculturalSciences,Nanjing２１００１４,China;∗Correspondingauthors,EＧmail:wlhyang＠sohuc．om,fangjc＠１２６c．om)WANGLihua,SHANDan,FANGJichao．ExpressionprofilingandfunctionofLHSC７０inLaodelphaxstriatellus．ChinJRice
3、Sci,２０１５,２９(４):４２４Ｇ４３０．Abstract:LaodelphaxstriatellusisanmajorpestonriceinChina．Ithasstrongadaptabilitytoenvironmentsandwasfoundinallricegrowingareasofthecountry．Asamolecularchaperone,HSP７０playsanimportantroleinbiologicalgrowthandresponsetostress．HSC７０,characterizedbyconstit
4、utiveexpression,isoneofthemostimportantmembersoftheHSP７０family．ToexploretheadaptationmechanismsofL．striatellustoenvironments,LHSC７０expressionprofilingandfunctionwereanalyzed．TheresultsshowedthecompletecDNAofLHSC７０is１９７１bpinlength,whichencodesaproteinof６５６aminoacids,withamole
5、cularweightof７１．６kDandtheoreticalisoelectricpointof５．４６．LHSC７０containsthreesignaturesequencesofHSP７０familyandcarriesatypicalHSP７０familystructuralsignature．Thephylogenictreeshoweditshares９９％homologywithHSC７０ofNilaparvatalugens．TheexpressionofLHSC７０wasassociatedwithlarvalinsta
6、r．TheexpressionlevelofLHSC７０washighestinfemaleadultsandwassimilarinmaleadultsandlarvaeatvariousinstars．Thedifferenceintheexpressionofthegenewasnotsignificantafter１０,１６,２２,２６,３０,３４,３８and４２℃treatments．DownregulatedexpressionlevelofLHSC７０duetoRNAisignificantlydecreasedthetolera
7、nceofthefemaletoheatstress,whileexertedalessinfluenceontheoviposition．TheseresultssuggestedthatLHSC７０isa７０kDheatshockcognateproteinandisnotinducedbyheatstress,butitcontributestheconstitutiveresistancetoheatwithoutcloserelationtoreproductioninL．striatellus．Keywords:Laodelphax
8、striatellus;HSC７０;expression;RNAi;constitutiveresistance王利华,单丹,方继朝．灰飞虱LHSC７
