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1、作物学报ACTAAGRONOMICASINICA2014,40(7):1205−1212http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2014.01205桑树EIN2基因的分离与表达1111112刘长英吕蕊花朱攀攀范伟李军王晓红李镇刚1111,*王茜龄赵爱春鲁成余茂德12西南大学生物技术学院/西南大学家蚕基因组生物学国家重点实验室,重
2、庆400715;云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101摘要:EIN2是植物体内乙烯信号转导的中心元件,负责将乙烯信号由内质网传递到细胞核中。本文通过检索桑树基因组数据,获得一个EIN2候选基因(MaEIN2),并进行生物信息学分析和表达分析。MaEIN2全长5614bp,由7个外显子和6个内含子组成,包含3921bp的CDS,编码1036个氨基酸残基。MaEIN2在进化树中与草莓、桃树等双子叶植物的EIN2蛋白关系较近,与单子叶植物关系较远。MaEIN2在老叶和成熟果实中的表达量分
3、别高于在幼叶和幼果中,且随果实发育呈逐渐上升趋势,MaEIN2可能与器官的成熟衰老有关。选用乙烯利、ABA和NaCl处理桑树种苗,乙烯利能够促进MaEIN2的表达,而ABA和NaCl抑制MaEIN2的表达。本文为深入研究MaEIN2基因的功能奠定了基础。关键词:桑树;MaEIN2;表达分析;乙烯利;ABA;NaClIsolationandExpressionofMulberry(MorusalbaL.)EIN2Gene1111112LIUChang-Ying,LÜRui-Hua,ZHUPan-P
4、an,FANWei,LIJun,WANGXiao-Hong,LIZhen-Gang,1111,*WANGXi-Ling,ZHAOAi-Chun,LUCheng,andYUMao-De12CollegeofBiotechnology,SouthwestUniversity/StateKeyLaboratoryofSilkwormGenomeBiology,Chongqing400715,China;InstituteofSericultureandApiculture,YunnanAcademyo
5、fAgriculturalSciences,Mengzi661101,ChinaAbstract:EIN2isacentralcomponentofethylenesignalingpathway,transferringtheethylenesignalfromendoplasmicreticu-lumtonucleus.AputativeEIN2geneobtainedfromMorusGenomeDatabaseanditsexpressionlevelsweredetectedbyusi
6、ngRT-PCR.Thefull-lengthgenomicsequenceofMaEIN2is5614bpinlength,whichconsistsofsevenexonsandsixintrons.TheCDSofMaEIN2is3921bpandencodes1036aminoacids.PhylogenetictreeanalysisrevealedthatMaEIN2hadcloserrelation-shipswithdicotyledonsplantsandfurtherwith
7、monocotyledonsplants.TheexpressionlevelsofMaEIN2inoldleafandmaturefruitweremuchhigherthantheseinyoungleafandimmaturefruit.TheexpressionlevelsofMaEIN2wasincreasinggraduallyinfruitdevelopment,indicatingMaEIN2wasinvolvedintheripeningandsenescenceofplant
8、organs.Ethephon,ABAandNaClwereusedtoincubatethemulberryseeds,showingthatethephoninducedtheexpressionofMaEIN2inseedlings,whileABAandNaCldownregulatedtheexpressionofMaEIN2.ThisworklaysafoundationforfurtherunderstandingofthefunctionofMaEIN2.Keywords:Mul
