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1、BacterialCultivationChenQingxinDepartmentofimmunologyandmicrobiologyprincipalToobtaininformationofbacterialbiologiccharacteristics,itisnecessarytoobservebacteriainculture.Inordertocultivatethemsuccessfully,wemustprovidethemwithsuitablenutrients:protein,carbohydrates,vitamins,andsoo
2、n.Culturemedium:Isthemixtureofvariousnutrientsthatissuitableforthegrowthofmicroorganisms.TypesofculturemediaBasedonthefunctionandchemicalcomponentsBasedonthephysicalstateBasedonthefunctionandchemicalcomponentsBasicmediumEnrichedmediumSelectivemediumDifferentialmediumBasedonthephysi
3、calstateLiquidmediumWithoutagarFortheproliferationofbacteriaSolidmedium1.5-2.5%agarFortheisolationandidentificationofbacteria,preservationofbacteriaE.g..SlantandplatesSemisolidmedium0.3-0.5%agarFortheobservationofbacterialmotilityInoculationandtransfertechniques methods:Streak-plat
4、etechniqueSlantinoculationLiquidmediuminoculationSemisolidmediuminoculation1.streak-platetechniqueMaterials(streakplate)CulturemediumAgarplateBacterialstrainsAmixtureofS.aureus&E.coli(金大混合菌)Inoculateloop,alcohollamp,markingpencil,andsoonInoculationloopisheateduntilitisred-hotTubeen
5、closureisremovedmouthoftubeisflamedAloopfuloforganismisremovedfromtubeLoopisremovedfromthecultureandtubeisflamedTubeenclosureisreturnedtubeFour-areastreakplatetechniquewithgentlypressure,don’tscratchthesurfaceoftheagarBacterialgrowthpatternsInagarplatemediumConfluentgrowth/smear;Co
6、lony:Aclusterofmicroorganismsgrowingonasolidmedium.Itisdirectlyvisibleandarisesfromasinglecell.FortheisolationofbacteriaConfluentgrowthatbeginningofstreakIsolatedofcoloniesattheendofstreakAtleasttwotypesofcoloniesDescribethesize,shape,pigment,margin,andsoon2.SlantinoculationMateria
7、lsCulturemediumslantmediumBacterialstrainsE.coli(大),Shigella(痢),S.aureus(金),anyoneInoculateloop,alcohollamp,markingpencil,andsoonSlantinoculationStreakastraightlineonthesurfaceoftheslant,thenstreakSlineonthesurfaceofslantwithgentlypressureBacterialgrowthpatternsInslantmdiumUniforml
8、awnForpreservationofbacter
