间歇低氧对大鼠 INS-1细胞 JNK、PDX-1的影响.pdf

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1、·56·解放军医药杂志2014年7月第26卷第7期Med＆Ph!』：：!：!·论著·间歇低氧对大鼠INS一1细胞JNK、PDX一1的影响卢巍，于娜，林雪娇，王东亮，康健[摘要]目的体外培养大鼠INS一1细胞，观察间歇低氧条件直接作用于细胞引起的改变。方法常氧组细胞(CON)不进行间歇低氧暴露，IH组细胞暴露间歇低氧，收集暴露(1、2、3d)的培养上清及细胞，检测氧化应激指标超氧化物歧化酶(SOD)、丙二醛(MDA)及JNK1mRNA、JNK1／2蛋白表达水平。给予SP600125阻断JNK蛋白活性，观察下游基因P

2、DX一1mRNA及蛋白变化。结果随着间歇低氧暴露时间的延长，IH组细胞培养上清中MDA含量呈增高趋势，SOD活力呈降低趋势。IH组细胞表达JNK1mRNA较相应时间CON组明显增高，其表达量进行性下降。磷酸化JNK1于IH3d组呈现出高表达。给予SP600125可引起PDX．1蛋白明显增高。结论间歇低氧可以直接引起INS一1细胞氧化损伤，随暴露时间的延长而加重，可能是通过诱导JNK1转录水平及蛋白活化而实现的。[关键词]细胞低氧；氧化性应激；胰岛素分泌细胞；大鼠[中国图书资料分类号]R一332[文献标志码]A[文

3、章编号]2095—140X(2014)07—0056-05『DoI110．3969／j．issn．2095．140X．2014．07．014EffectofIntermittentHypoxiaonJNKandPDX-1ofINS-1PancreaticBetaCellsinRatsLUWei，YUNa，LINXue—jiao，WANGDong—liang，KANGJian(1．DepartmentofRespiratoryDiseases，theFirstAfiliatedHospitalofChinaMedi

4、calUniversity，Shenyang110001，China；2．DepartmentofRespiratoryDiseases，202HospitalofPLA，Shenyang110003，China)[Abstract]ObjectiveToobservethedirectefectonINS一1cellsofratsculturedinvitrounderintermittenthy—poxia．MethodsCellsincommonoxygengroup(CONgroup)wasnotexpo

5、sedunderintermittenthypoxia，whileceilsinintermittenthypoxiagroup(IHgroup)wereexposedunderintermittenthypoxia．Cuhuredsupernateandcellswerecol—lectedat1d，2“dand3dafterexposureandthelevelsofsuperoxidedismutase(SOD)，malondialdehyde(MDA)andexpressionsofJNK1mRNAand

6、JNK1／2inthetWOgroupsweredetected．SP600125wasusedtobolcktheactivityofJNK，andchangesofdownstreamgenePDX一1mRNAandproteinwerealsoobserved．ResultsTheMDAlevelatsuper—natewassignificantlyhigherandSODactivitywassignificantlydecreasedinculturedsupernateofIHgroupwithpr

7、olongedexposuretimeunderintermittenthypoxia．JNK1mRNAexpressionwassignificantlyupregulatedinIHgroupcomparedwiththatinCONgroupatthesametime，andexpressionvaluewasprogressivelydecreased．Thehighphospho—JNK1ex—pressionwasfound3dafterexposure．TheexpressionofPDX一1was

8、significantlyincreasedafterusingSP600125．Con—clusionIntermittenthypoxiamaydirectlyinduceoxidativedamageeffectonINS一1cells，andthedamagebecomesseri—OUSwithtimeprolonging，anditsdamagemaybedo