翘嘴红鲌热休克蛋白60的基因克隆及其表达特征分析.pdf

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1、第35卷第2期2016年3月浙江海洋学院学报(自然科学版)JournalofZhejiangOceanUniversity(NaturalScience)V01．35No．2Mar．，2016文章编号：1008—830X(2016)02—0105—08MolecularCloning，CharacterizationandExpressionAnalysisofHeatShockProtein60inErythroculterUishaeformisDAIDong，YANGYu-hong(CollegeofAnimalScienceandT

2、echnology，NortheastAgriculturalUniversity，Harbin150030，China)Abstract：Intllepresentstudy．thecDNAofheatshockprotein60fromErythrocuherilishaeformis(EiH-SP60)wasclonedbythecombinationofhomologycloningandrapidamplificationofcDNAends(RACE)ap—proaches．711lefull—lengtheDNAofEiHSP

3、60wasof2323bp。includinganopenreadingframe(ORF)of1728bpencodingapolypeptideof575aminoacidswithpredictedmolecularweightof61．26kDaandtheoreticalisoeleetricpointof5．27．BLASTanalysisrevealedthatEiHSP60sharedhishsimilaritywithotherknownHSP60s，andoneconservedChaperonin60signature

4、wasalsoidentifiedinEiHSP60，whichindicatedthatEiHSP60shouldbeamemberoftheHSP60family．ThemRNAofEiHSP60Wasconstitutivelyexpressedinalltestedtissuesofuntreatedtopmouthcuher，includingbrain，muscle，kidney，liver，skin，spleen，heart，gillandintestinewiththehighestexpressionlevelinthel

5、iver．Fluorescentreal—timequantitativeRT-PCRWasusedtoexaminetheexpressionoftheEiHSP60geneintopmouthculterafterthechallengewithU)S．Acleartime—de-pendentexpressionpatternofEiHSP60wasfoundaftertheU玛challenge．andtheup-regulatedmRNAex—pressionofEiHSP60intopmouthcuherafterU)Schal

6、lengeindicatesthattlleHSP60geneiSinducibleandin-volvedinimmuneresponses．TheseresultssuggestthatEiHSP60playsanimportantroleinimmuneresponsesintopmouthculter．‘Keywords：Erythroculterilishaeformis；heatshockprotein60；genecloning；characterizationanalysisCI。Cnumber：S9】7．4Document

7、code：A翘嘴红鲔热休克蛋白60的基因克隆及其表达特征分析戴东，杨雨虹(东北农业大学动物科技学院，黑龙江哈尔滨150030)摘要：利用同源克隆和RACE技术克隆了翘嘴红鲐热休克蛋白HSP60eDNA全长序列，并对HSP60基因的序列特征进行了分析。EiHSP60eDNA序列长2323bp，开放阅读框为1728bp，编码575个氨基酸，其编码蛋白相对分子量为Receiveddate：2016--01-10收稿日期：2016—0l—lOFundproject：TheProgramforHeilongjiangscienceandtechnol

8、ogy(GA068203_4—3)基金项目：黑龙江省科技计划项目(GA068203--4-3)Biography：DAIDang(1992-)，Male，ShandongZaoz