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1、目录中文摘要英文摘要符号说明-•、几—刖B研究对象和方法结果10讨论141819结论不足与展望参考文献20致谢23综述25CONTENTSChineseabstract1Englishabstract2Symbolillustratinns3Background4Patientsandmethods5Results10Disscussion14Conclusion18Deficiencyandprospect19References20Acknowledgements23Review25GP73
2、联合AFP、VEGF在原发性肝癌的诊断中的价值探讨摘要冃的研究高尔基体糖蛋白-73(GP73)、甲胎蛋白(AFP)、血管内皮生长因子(VEGF)在原发性肝癌(PHC)患者血清中的表达意义。方法43例PHC及9例肝良性肿瘤患者接受双抗体夹心ELISA法检测血清GP73、VEGF浓度及电化学发光法检测血清AFP浓度。结果GP73在不同年龄、性别、肿瘤大小及乙肝病毒携带者的表达无明显差异(P>0.05)oGP73浓度肝硬化组高于无肝硬化组［(252.977±86.985)vs(196.697±90.8
3、47)ng/mL,P<0.05］,PHC组高于肝良性肿瘤组［(241.413+77.079)vs(101.866±74.192)ng/mL,PC0.01］。手术前组GP73、AFP和VEGF表达水平均高于手术后组［(674.176±1090.083)vs(178.560±289.330)ng/mL,(256.666±164.76)vs(149.072±158.643)ng/mL,PV0.01］。绘制AFP、GP73、VEGF在PHC鉴别诊断的ROC曲线，曲线下面积分别为0.894、0.791、0
4、.612,GP73面积为最大。GP73诊断PHC组敏感性及特异性为88.4%、77.7%,高于AFP。将AFP及GP73并联检测后发现，敏感度为96.8%,特异度为63.7%,与单项检测相比，GP73联合AFP可明显提高PHC诊断的准确性。结论GP73是一种灵敏度、特异度更高的PHC诊断的血清标志物，而GP73、AFP联合检测可提高PHC诊断准确性。关键词：高尔基体糖蛋白-73；甲胎蛋白；血管内皮生长因子；原发性肝癌ThediagnosisvalueofGolgi-protein73,Alpha
5、-fetoproteinsandVascularendothelialgrowthfactorinprimaryhepaticcancerAbstratObjectiveToinvestigatetheexpressionandclinicalsign讦icanceofserumGolgiprotein73,Alpha-fetoproteins(AFP)andVascularendothelialgrowthfactor(VEGF)inprimaryhepaticcancer(PHC)patie
6、nts.MethodsSerumAFP,GP73andVEGFlevelsof43PHCpatientsand9benignhepatictumorpatientsweredetectedbyelectrochemiluminesceneeanddoubleantibodysandwichELISA.ResultsTheserumlevelsofGP73indifferentages,genders,tumorsizes,hepatitisBviruscarrierswasnotsignific
7、antlydifferent(P>0.05).TheserumlevelsofGP73inhepaticcirrhosisgroupwassignificantlyhigherthannociiThosisgroup](241.413+77.079)vs(101.866+74.192)ng/mL,P<0.01].TheserumlevelsofGP73inPHCgroupwassignificantlyhigherthanbenigntumorofliverginup[(241.413+77.0
8、79)us(101.866±74.192)ng/mL,P<0.01].TheserumlevelsofGP73,AFPandVEGFinpreoperativegroupweresignificantlyliigherthanthatinpostoperativegroup[(674.176±1090.083)vs(178.560±289.330)ng/mL,(256.666±164.76)vs(l49.072±158.643)ng/mL,allP<0.01].TheareaofAFP,GP73
