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时间:2020-03-25
《猪传染性胸膜肺炎的ApxIV-ELISA检测方法.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、南方农业学报JOURNALOFSOUTHERNAGRICULTURE2013,44(12):2098—2101ISSN2095—1191;CODENNNXAABhttp://www.nfnyxb.cnDOI:10.39696:issn.2095-1191.2013.12.2098猪传染性胸膜肺炎的ApxIV—ELISA检测方法李凤梅,龙小曾,李崇,谢娟,苏亚权,林昌华,钟诚+(广西大学动物科学技术学院,南宁530005)摘要:【目的】建立一种敏感性好、简便快捷,适用于临床诊断、实验室检测和血清学调查的诊断技术,为规模化养猪场监控胸膜肺炎放线
2、杆菌(APP)感染及净化猪传染性胸膜肺炎(PCP)提供技术支撑。【方法】以ApxⅣ重组蛋白为抗原,建立ApxIV.ELISA检测方法,经可行性、特异性、准确性、重复性检验后,对采自广西南宁周边地区随机抽取的未免疫APP疫苗育肥猪与经产母猪进行血清抗体检测。【结果】经试验证实,1)2ApxW.ELISA检测APP具有可行性,且具有特异性良好、准确性较高、重复性较好的特点,其判断标准:s(样品孔0D。。)>t0.397为阳性,S<0.397为阴性。以ApxIV—ELISA检测的90份血清样品中有40份为阳性,阳性率为44.4%;其中经产母猪阳性
3、血清33份,占总阳性血清的82.5%。【结论】广西南宁周边地区的养猪场已普遍存在APP感染,特别是经产母猪较严重。采用ApxlV—ELIsA检测方法能及时鉴别诊断猪群的APP感染情况,且敏感性好、简便快捷,适用于临床诊断、实验室检测和血清学调查。关键词:猪传染性胸膜肺炎;胸膜肺炎放线杆菌;经产母猪;阳性率;ApxIV—ELISA中图分类号:$854,43文献标志码:A文章编号:2095—1191(2013)12—2098—04ApxlV-ELISAdetectionmethodforporcinecontagiouspleuropneumo
4、niaLIFeng—mei,LONGXiao—zeng,LIChang,XIEJuan,SUYa—quan,LINChang-hua,ZHONGCheng+(CollegeofAnimalScienceandTechnology,GuangxiUniversity,Nanning530005,China)Abstract:【Objective]InordertoprovidetechnicalsupportfordetectingandcontrollingActinobaeilluspleuropneumoniae(APP)andpuri
5、fyingPleuropneumoniaeinlarge-scalepigfarms,weestablishedflsensitiveandconvenientdiagnostictechniqueforclinicaldiagnosis,laboratorytestingandserologicalsurvey.【Method】SettingupApxIV—ELISAdiagnosticmethodbythefeasibility,specificity,accuracyandrepeatabilitytestwithApxIVrecom
6、binantprotein,serumantibodiesofno—immunizedpleuropneumoniavaccinefatteningpigsandmuhiparousSOW,whichcamefrompigfarmsinNanningareaofGuangxi,weredetected.【Result】TheexperimentverifiedthatApxIV—ELISAdiagnosticmethodwasfeasibletodetectAPP.Themethodhasgoodspecificity,highaccura
7、cy,andgoodrepeatability.Thecriteriafordeterminationwereasfallows.When0D630am(Samplehole)wasgreaterthanorequaltoO.397,theselqlmwasidentifiedaspositive.When0D630amwaslessthan0.397,theserumwasidentifiedasnegative.IntheninetyseFilmsamples。fortysenlmsampleswerepositiveandthepos
8、itiveratewas44.4%.Inthefortypositiveserumsamples.thirty-threeserumsamplescamefromthemohip
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