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1、22.Thechemicaltoolofclaim21whereintherestrictionendonucleasefortargetingDNAsequencesisselectedfromthegroupconsistingof:SequencecutEnzymeNameSEQIDNO:AAR/AATTYAcsI53R/AATTYApoI53A/AGCTTHindIII54AA/CGTTPsp1406I55AAT/ATTSspI56ACA/CRYGTAflIII57A/CCGGTAgeI58A/CATGTBspLU11I59ACCTGC(4,8)
2、BspMI60,61ACTGG(1,�6�11)BsrI62,63R/CCGGYBsrFI64R/CCGGYCfr10I64A/CGTMaeII65A/CGCGTMluI66RCATG/YNspI67A/CCGGTPinAI58A/CCWGGTSexAI68A/CTAGTSpeI69AGAGG/CCTAatI70AG/CTAluI71A/GATCTBglII72R/GATCYBstYI73RG/GNCCYDraII74AGC/GCTEco47III75RG/GNCCYEcoO109I74RGCGC/YHaeII76RG/GWCCYPpuMI77AGT/A
3、CTScaI78AGG/CCTStuI70R/GATCYXhoII73ATAT/TAATAseI79AT/TAATAsnI79AT/CGATBspDI80AT/CGATClaI80ATGCA/TNsiI81ATTT/AAATSwaI82CACAGNNN/CTGAlwNI83CAC/GTGBbrPI84CACNNN/GTGDraIII85YAC/GTRBsaAI86C/AATTGMfeI87C/AATTGMunI87CA/TATGNdeI88CATG/NlaIII89CMG/CKGNspBII90CAC/GTGPmaCI84CAC/GTGPmlI84CAG
4、/CTGPvuII91CR/CCGGYGSgrAI92(13,9)CATCCFokI93,94CCC/CGCAciI95CC/TNAGGAocI96/CCWGGApyI97C/CTAGGAvrII98C/CTAGGBinI98C/CNNGGBsaJI99CCNNNNN/NNGGBsiYI23CCNNNNN/NNGGBslI23CCANNNNN/NTGGBssGI27CC/WGGBstNI100CCANNNNN/NTGGBstXI27CC/TNAGGBsu36I96C/CRYGGDsaI101C/YCGRGAvaI102CCTNN/NNNAGGEcoNI3
5、2/CCWGGEcoRII97C/CGGHpaII103CCGC/GGKspI104CCTC(7,6)MnlI105,106C/CGGMspI103CC/TNAGGMstII96CC/WGGMvaI100CC/SGGNciI107C/CATGGNcoI108CMG/CKGNspBII90CCANNNN/NTGGPflMI44CCGC/GGSacII104CC/TNAGGSauI96CC/NGGScrFI109CCC/GGGSmaI110CCTGCA/GGSse8387I111CCGC/GGSstII104C/CWWGGStyI112CCANNNN/NTG
6、GVan91I44CCANNNNN/NNNNTGGXcmI50C/CCGGGXmaI113C/CCGGGXmaCI113(1,�6�11)CCAGTBsrI114,115CG(10,12)CGANNNNNNTGC(12,10)BcgI116,117CGRY/CGBsiEI118C/GTACGBsiWI119CG/CGBstUI120C/GGCCGEagI121Y/GGCCRCfrI122Y/GGCCREaeI122C/GGCCGEclXI121CGTCTC(1,5)Esp3I123,124CG/CGFnuDII120CG/CGMvnI120CGAT/CG
7、PvuI125CG/GWCCGRsrII126CR/CCGGYGSgrAI92CG/CGThaI120C/GGCCGXmaIII121CTC/TTAAGAflII127C/TAGBfaI128C/TTAAGBfrI127CTGGAG(16,14)BpmI129,130C/TNAGDdeI131CTCTTC(1,4)EarI132,133C/YCGRGAvaI102CTGAAG(16,14)Eco57I134,135CTGGAG(16,14)GsuI129,130C/TAGMaeI128C/TCGAGPaeR7I136CTGCA/GPstI137C/TAG
8、RmaI128C/TRYAGSfcI138C/TCGAGXhoI136(14,1
