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1、在蛋门质组学研究策略的能力和局限的电泳分离PowerandlimitationsofelectrophoreticseparationsinproteomicsstrategiesThierry.Rabilloud1,2,AliR.Vaezzadeh3,NoellePotier4,CecileLelong1,5,EmmanuelleLeize-Wagner4,MireilleChevallet1,21：CEA,IRTSV,LBBSI,38054GRENOBLE,France.2:CNRS,UMR5092,Bio
2、chimieetBiophysiquedesSystemesIntegres,GrenobleFrance3:BiomedicalProteomicsResearchGroup,CentralClinicalChemistryLaboratory,GenevaUniversityHospitals,Geneva,Switzerland4:CNRS,UMR7177.InstitutdeChimedeStrasbourg,Strasbourg,France5:UniversiteJosephFourier,Gren
3、obleFranceCorrespondence:ThierryRabilloud,iRTSV/LBBSI,UMRCNRS5092,CEA-Grenoble,17ruedesmartyrs,F-38054GRENOBLECEDEX9Tel(33)-4-38-78-32-12Fax(33)-4-38-78-44-99e-mail:Thierry.Rabilloud@cea.frAbstract:Proteomicscanbedefinedasthelarge-scaleanalysisofproteins.Due
4、tothecomplexityofbiologicalsystems,itisrequiredtoconcatenatevariousseparationtechniquespriortomassspectrometry.Thesetechniques,dealingwithproteinsorpeptides,canrelyonchromatographyorelectrophoresis.Inthisreview,theelectrophoretictechniquesareunderscrutiny.Th
5、eirprinciplesarerecalled,andtheirapplicationsforpeptideandproteinseparationsarepresentedandcriticallydiscussed.Inaddition,thefeaturesthatarespecifictogelelectrophoresisandthatinterplaywithmassspectrometry(i.e.,proteindetectionafterelectrophoresis,andtheproce
6、ssleadingfromagelpiecetoasolutionofpeptides)arealsodiscussed.Keywords:electrophoresis,two・dimensionalelectrophoresis,isoelectricfocusing,immobilizedpHgradients,peptides,proteins,proteomics.TableofcontentsI.IntroductionILTheprinciplesatplayIII.Howtouseelectro
7、phoresisinaproteomicsstrategyIII.A..Electrophoreticseparationofpeptides.III.A.1.CapillaryIEFIII.A.2.SolutionIEFIII.A.3.IPG-IEFIII.A.4.Off-GelIII.A.5.AnewdimensionfordatavalidationIII.B.Electrophoreticseparationofproteins.III.B.l.Electrophoreticseparationsofn
8、ativeproteinsIII.B.2.Electrophoreticseparationsofdenaturedproteins-rationaleIII.B.3・TheimplementationofdenaturingproteinelectrophoresisinproteomicsIII.B.4.Theworkhorseofproteinseparationinproteo
