孕期亚临床甲减大鼠模型的建立

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1、孕期亚临床甲减大鼠模型的建立于晓会1,2,朱帅俊1,2,刘迪杰⑷单忠艳1・2,滕卫平】.2(1.中国医科大学附属第一医院,沈阳110001；2.辽宁省内分泌疾病重点实验室，沈阳110001)摘要：目的建立可靠的孕期亚临床甲减大鼠动物模型。方法：将60只成年雌性Wistar大鼠随机分为三组：①完全甲减组：手术完全切除甲状腺；②亚临床甲减组：手术完全切除甲状腺后，予L-T4微渗泵l.Opg/(lOOgd)浓度持续皮下泵入，H怀孕第15天剂量改为1.05曲/(lOOg-d)；③止常对照组：行甲状腺假手术。各组大鼠在基础状态、孕前和怀孕后第10、13、17天利分娩当天采血测定甲状腺功能。结果：各组人

2、鼠在基础状态时，血清TSH和TT4水平无统计学弟异。完全卬减组大鼠在孕前和整个怀孕过程中及分娩当天血清TSH水平始终明显高于亚临床甲减组和正常对照组(都有PvO.Ol),血清TT4水平始终明显低于其他两组(都有PvO.Ol)。亚临床甲减组大鼠在孕前和整个怀孕过程中及分娩当天血清TSH水平始终明显髙于止常对照组(都有PvO.05),血淸TT4水平始终与止常对照组相当(都有P>0.05)o结论：应用于-术完全切除甲状腺，再予微渗泵皮下持续输入合适剂量的L-T4可以成功建立孕期亚临床甲减大鼠模型。关键词：内科学；亚临床卬减；孕期；大鼠模型中图分类号:R581.2Establishmentofani

3、malmodelofpregnantratwithsubclinicalhypothyroidismYUXiaohuii,2,ZHUShuaijum.2,LIUDijiei.2,SHANZhongyam.2,TENGWeipingi.2(1.TheFirstHospitalofChinamedicalUniversity,ShenYang110001;2.KeyLaboratoryofEndocrineDiseasesofLiaoningProvince,ShenYang110001)Abstract:ObjectiveToestablishthebelievableanimalmodelo

4、fpregnantratwithsubclinicalhypothyroidism・Methods:60femaleadultWistarratweredividedintothreegroups:①overthypothyroidism(OH)group:totalremovaloftherats'thyroid.②subclinicalhypothyroidism(SCH)group:aftertotalremovaloftherats'thyroid,levothyroxineweregiventotheratsbyminiosmoticpumpsandtheratewas1.0pg/

5、(100gd).③controlgroup:thefalsethyroidsurgerywascaiTiedout.Theserumsampleswerecollectedfromallthethreegroupsatbaseline,preconception,1Oth,13th,17thgestationaldaysandatdelivery.Thyroidfunctionwasdetectedatallthetimepoints.Results:Atbaseline,thyroidfunctionindexeslevelsweresinlilaramongthethreegroups・

6、Afterthyroidsurgery,TSHlevelsofOHgroupwerealwayshigherthanthoseofSCHgroupandcontrolgroup(allP<().()1).AndTT4levelswerealwayslowerthanthoseofothertwogroups(allP<0.01).InSCHgroup,frompreconceptiontodeliveryserumTSHlevelswerealwayshigherthanthoseofcontrolgroup(allP<0.05).SerumTT4levelswerealwayssimila

7、rwiththoseofcontrolgroup(allP>0.05).Conclusion:Aftertotalremovalthyroidandoptimaldoseoflevothyroxineinfusionwithminiosmoticpump,asuccessfulanimalmodelofpregnantratwithsubclinicalhypothyroidismcanbeestablish