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《Stimulation of Cyclic ADP-ribose Synthesis by Acetylcholine and Its乙酰胆碱及其对ADP-核糖合成的刺激作用 牛肾上腺嗜铬细胞儿茶酚胺释放的作用》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、THEJOURNALOFBIOLOGICALCHEMISTRYVol.272,No.34,IssueofAugust22,pp.21002±21009,1997©1997byTheAmericanSocietyforBiochemistryandMolecularBiology,Inc.PrintedinU.S.A.StimulationofCyclicADP-riboseSynthesisbyAcetylcholineandItsRoleinCatecholamineReleaseinBovin
2、eAdrenalChromaffinCells*(Receivedforpublication,October11,1996,andinrevisedform,May15,1997)KatsuyaMorita,ShigeoKitayama,andToshihiroDohi³FromtheDepartmentofPharmacology,HiroshimaUniversitySchoolofDentistry,1-2-3Kasumi,Minami-ku,Hiroshima734,Japan21212
3、1CyclicADP-ribose(cADPR)issuggestedtobeanovelmobilizingintracellularCa:Ca-inducedCareleasemessengerofryanodinereceptorsinvariouscellular(CICR),mediatedbytheryanodinereceptors.CICRmediatessystems.However,theregulationofitssynthesisinre-theamplification
4、andpropagationofinitialCa21signals,thesponsetocellstimulationanditsfunctionalrolesaregenerationofCa21oscillations,andthepropagationofCa21stillunclear.Weexaminedthephysiologicalrelevancewavesincertaintypesofcells.Depolarizingstimulicanacti-ofcADPRtothe
5、messengerroleinstimulation-secretionvatereleaseofCa21fromryanodine-sensitiveintracellularcouplinginculturedbovineadrenalchromaffincells.storesinanumberofneuronalcells(1).Incerebellargranule2121SensitizationofCa-inducedCarelease(CICR)and1cells,amajorco
6、mponentofbothKdepolarization-andN-stimulationofcatecholaminereleasebycADPRinper-21methyl-D-aspartate-inducedelevationof[Ca]iappearstobemeabilizedcellsweredemonstratedalongwiththecon-21Downloadedfrom21duetothereleaseofCafromintracellularstores,evidence
7、tributionofCICRtointracellularCadynamicsand21thatsuggeststheimportanceofintracellularCareleaseviasecretoryresponseduringstimulationofintactchro-maffincells.ADP-ribosylcyclasewasactivatedintheCICR(2).21membranepreparationfromchromaffincellsstimu-Galion
8、eetal.(3)recentlyidentifiedanovelCamobilizinglatedwithacetylcholine(ACh),excessKCldepolariza-agentthatisacyclicmetaboliteofnicotinamideadeninedinu-1tion,and8-bromo-cyclic-AMP.ACh-inducedactivationcleotide(NAD),cyclicADP-ribose(cADPR).Thisagent
