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1、脂肪间充质干细胞相关分化与炎症相关威斯腾生物技术中心威斯腾生物十二周年庆技术培训之目录脂肪间充质干细胞分离、培养和鉴定脂肪间充质干细胞骨关节炎和肥胖脂肪间充质干细胞分化与炎症脂肪间充质干细胞(ASCs).Adiposetissueisrecognizedasacomplexendocrineorganthatplayscentralrolesinenergyhomeostasis,feeding,insulinsensitivity,andinflammation.Adiposetissuecontainsdifferentcelltypesbesidesa
2、dipocytes,includingadipose-derivedstromalcells(ASCs).ASCswerefirstreportedbyFrohlichin1972,asadherent,proliferatingadipocyteprecursors.Differentnameshavebeenusedtodescribetheadherentcellpopulationthatcanbeexpandedfromlipoaspirates,e.g.lipoblast,pericytes,preadipocytes,processedlipo
3、aspirates(PLA)cells,andmanyothers.Severalworksdemonstratedthestemcell-likeplasticityofASCsandtheircapabilityofdifferentiatingintocellsofmesodermalorigin,suchasadipocyte,osteocyte,chondrocyteandmyocytelineages.ZukPA,etc.Humanadiposetissueisasourceofmultipotentstemcells.MolBiolCell20
4、02;13:4279-4295PLAorSVF(stromalvascularfraction)Toclarifynomenclature,theInternationalFatAppliedTechnologySociety(IFATS)proposedtonameASCstheisolated,plastic-adherent,multipotentcellpopulationobtainedfromadiposetissuethroughdifferentmethodsSubcutaneousfatisanabundantandaccessibleso
5、urceofbothheterogeneousstromalvascularfraction(SVF)cellsandasmallnumberofrelativelyhomogeneousASCs.SVFcellsincludevascularendothelialcellsandtheirprogenitors,smoothmusclecells,immuneregulatorymonocytes/macrophagesandTregulatorycells.Caspar-Bauguil,S.,etal.,Adiposetissuelymphocytes:
6、typesandroles.JPhysiolBiochem,2009.65(4):p.423-36.Similarlytobonemarrowmesenchymalstromalcells(BMSCs),ASCswereidentifiedinvivoascellswithperivascularlocalization,inclosecontactwiththeothercelltypes.LikeotherMSCtypes,ASCscannotbeidentifiedbyasinglespecificmarker,butmultiplemarkercom
7、binationsareneededfortheidentificationofthecellpopulation.ASCsexpressthesurfacemarkersusedforBMSCcharacterization,withsomepeculiarities.BMSCsexpressCD106thatisnotorslightlyexpressedbyASCs;bycontrast,BM-MSCslacktheexpressionofCD49d,whichisexpressedbyASCs.Followingexvivoexpansion,som
8、emarkersarenormallyexpress
