高效液相色谱法测定丙戊酸钠的血药浓度

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1、·药物研究·2013年6月20日第22卷第12期DrugIdentificationChinaPharmaceuticalsVol．22，No．12，June20，2013高效液相色谱法测定丙戊酸钠的血药浓度胡琳(浙江省人民医院，浙江杭州310014)摘要:目的建立测定血清中丙戊酸(VPA)血药浓度的高效液相色谱法。方法血清用正己烷提取，α－溴苯乙酮为衍生试剂，环己烷羧酸为内标，采用ZorbbxSBC18柱(250mm×4．6mm，5μm)为分析柱;流动相为甲醇－水(79∶21，V/V)，检测波长为248nm，流速为1．0mL/min，设计个体化给药方案。结果内标环己

2、烷羧酸和丙戊酸的保留时间分别为6．3min和9．3min，质量浓度线性范围为12．5～200μg/mL(r=0．9999)，平均回收率为99．98%，日内、日间RSD均小于5．66%。结论该法快速、简单、准确性好，适用于临床进行丙戊酸的血药浓度监测。关键词:高效液相色谱法;丙戊酸;血药浓度;个体化给药中图分类号:R969．1;R969．3;R971+．6文献标识码:A文章编号:1006－4931(2013)12－0016－03HPLCMethodforDeterminationofSodiumValproateSerumConcentrationHuLin(Zheji

3、angProvincialPeoplesHospital，Hangzhou，Zhejiang，China310014)Abstract:ObjectiveToestablishtheHPLCmethodfortheserumconcentrationdeterminationofvalproicacid(VPA)andtoguidetheindividualizedmedicationregimenbyanalyingthetypicalcases．MethodsSerumwasextractedwithhexane．Withα－bromoacetophenonea

4、sthederivativereagentandcyclohexanecarboxylicacidastheinternalstandard，theZorbbxSB－C18column(250mm×4．6mm，5μm)wasadoptedastheanalyticalcolumn;themobilephasewasmethanol－water(79∶21，V/V)，thedetectionwavelengthwassetat248nm，theflowratewas1．0mL/min．Theabovementionedmethodwasusedtodetermineth

5、edozensofcasesusingsodiumvalproate．Fromthem，3typicalcaseswereselectedfordiscussingtheinfluencingfactorsoftheserumsodiumvalproateconcentrationanddesigningtheindividualizeddrugadministrationregimen．ResultsTheretentiontimeofinternalstandardcyclohexanecarboxyacidandVPAwas6．3minand9．3minresp

6、ectively，thelinearrangewas12．5－200μg/mL，theratioofthesodiumvalproatepeakheightandtheinternalstan-dardpeakheightshowedgoodlinearrelationwiththeconcentration(r=0．9999)．Theaveragerecoveryratewas99．98%，RSDofin-tra－dayandinter－daywaslessthan5．66%．Atthesametime，duringtheprocessofthepracticalm

7、easurementofserumdrugcon-centrations，itwasfoundedthatthesamedosageofsodiumvalproatecouldproducealargergapoftheblooddrugconcentrationindif-ferentindividuals．ConclusionThismethodisrapid，simple，accurateandsuitableforserumsodiumvalproateconcentrationmonitor-ing．Inordertoadjustthein