A Model for Recognition Scheme between Double Stranded DNA and Proteins英文资料

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1、StructureandConformationofNucleicAcidsandProtein-NucleicAcidInteractionsEditedbyM.SundaraiingamandS.T.RaoCopyright1975UniversityPark.PressBaltimoreAModelforRecognitionSchemebetweenDoubleStrandedDNAandProteinsS.H.Kim,J.L.Sussman,andG.M.ChurchABSTRACTislikelythattherearemany·modesbywhichDNAcanbereco

2、gnizedby;roteins.AmodelforthecomplexbetweenadoublestrandedDNAandantiparallel~-ribbonisproposedasapossiblemodeofrecognitionbetweenhblestrandedDNAandproteins.Inthismodel,thesymmetryelements,as'IIJ'cl]astherepeatdistancesoftwocomponents,coincide,thusprovidinganice.:omplementarycontact.Thecontactisocc

3、urringonthenarrowgrooveofDNA,dthepolarityofeachDNAstrandisantiparalleltothebackboneofthe;teJJtidewithwhichthecomplementaryhydrogenbondsareformed.TRODUCTION~recognitionbetweenDNAandproteinisoneofthefundamentalmolecular-:rocessesgoingoninallthelivingcells.ExamplesofsuchDNA-proteincom-3:!xesarenumer

4、ous:histone-DNAcomplex,repressor-DNAcomplex,restriction=yme-DNAcomplex,etc.ThespecificrecognitionbetweenadoublestrandedDNA(dsDNA)andateincanoccurintwoways:onecase,inwhichdsDNAopensupandthe:aesequenceofthe'singlestrandedregionisrecognizedbytheprotein,andthe•ercase,inwhichthebase-paireddoublestrandi

5、tselfisrecognized.Themodelposedisageneralschemeforthelattertypeofrecognition.CarterandKraut(I)proposedamodelforadoublestrandedRNA(dsRNA)atwo-strandedantiparallel~structurewherethe2'hydroxylofribosein571572Kim,Sussman,andChurchRNAformsahydrogenbondtothefreecarbonyloxygenofthebackboneandthefreeNHgro

6、upformstwohydrogenbondswiththeringoxygenandthe2'hydroxyloxygenofthenextmoleculethroughawatermoleculeonthenarrowgrooveofthedsRNA.TheyalsopointedoutthatbecausethenarrowgrooveofthedsRNAissoshallow,thereisnoroomfora-carbonsintheantiparallel~structuretohaveanyresiduesotherthanverysmallsidechaingroups.E

7、venthoughdsDNAcanassumeadsRNAtypeofconformation,thisschemeisinapplicabletodsDNAwhichdonothavethe2'hydroxylgroup.TheproposedmodelinthispapercontainsthefeaturewhichallowsthenarrowgrooveofdsDNAtoberecognizedbyvariou