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1、ThisarticleappearedinajournalpublishedbyElsevier.Theattachedcopyisfurnishedtotheauthorforinternalnon-commercialresearchandeducationuse,includingforinstructionattheauthorsinstitutionandsharingwithcolleagues.Otheruses,includingreproductionanddistribution
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3、mationregardingElsevier’sarchivingandmanuscriptpoliciesareencouragedtovisit:http://www.elsevier.com/copyrightAuthor'spersonalcopyScientiaHorticulturae128(2011)408–414ContentslistsavailableatScienceDirectScientiaHorticulturaejournalhomepage:www.elsevier
4、.com/locate/scihortiProductionoftransgenicsweetpotatoplantsresistanttostemnematodesusingoryzacystatin-IgeneShangGaoa,1,BoYua,1,LiYuana,HongZhaia,b,Shao-zhenHec,Qing-changLiua,b,c,∗aKeyLaboratoryofCropGenomicsandGeneticImprovement,MinistryofAgriculture,
5、ChinaAgriculturalUniversity,No.2YuanmingyuanWestRoad,Beijing100193,ChinabBeijingKeyLaboratoryofCropGeneticImprovement,ChinaAgriculturalUniversity,No.2YuanmingyuanWestRoad,Beijing100193,ChinacLaboratoryofCropHeterosisandUtilization,MinistryofEducation,C
6、hinaAgriculturalUniversity,No.2YuanmingyuanWestRoad,Beijing100193,ChinaarticleinfoabstractArticlehistory:Stemnematode(DitylenchusdestructorThorne)isoneofmostseriousdiseaseslimitingsweetpotatoReceived11October2010(Ipomoeabatatas(L.)Lam.)production,andit
7、isurgenttodevelopsweetpotatovarietiesresistanttothisReceivedinrevisedform18February2011disease.Inpresentstudy,wehavedevelopedtransgenicsweetpotato(cv.Xushu18)plantsresistanttoAccepted20February2011stemnematodesusingoryzacystatin-I(OCI)genewithAgrobacte
8、riumtumefaciens-mediatedtransforma-tion.A.tumefaciensstrainEHA105harborsabinaryvectorpCAMBIA1301withOCIgene,uidAgeneandKeywords:hptIIgene.Selectionculturewasconductedusing7mg/lhygromycin.Atotalof2119plantswerepro-Agrobacteriumtumefacien