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1、茶叶科学2008，28（4）：301~308JournalofTeaScience叶绿素仪（SPAD）在茶树氮素营养诊断中的适用性研究1,2222*1*杨亦扬，马立锋，石元值，阮建云，黎星辉（1.南京农业大学茶叶研究所，江苏南京210095；2.农业部茶叶化学工程重点实验室，中国农业科学院茶叶研究所，浙江杭州，310008）摘要：测定叶片氮素含量是诊断茶树氮素状况的一项重要方法。本文利用盆栽试验，通过不同的供氮水平，研究了利用叶绿素仪（SPAD-502）进行茶树氮素营养快速诊断的适用性。结果表明，茶树新梢产量与氮素用量、成熟叶氮素含
2、量之间呈线性加平台的反应关系，成熟叶的SPAD值与其全氮含量呈显著线性正相关，并与茶树产量存在着比较明显的关系拐点，初步显示SPAD可以较好地表征茶树的氮素营养状况，有可能作为快速诊断方法用于指导茶树施肥，但是还需要通过大田试验进行进一步验证。本文还探讨了测定位点、叶位、天气状况、田间原位或离体测定方式、表面清洗以及样本量等因素对SPAD测试精度的影响。关键词：茶树；叶绿素仪；氮素营养；影响因素中图分类号：S571.1；S143.1文献标识码：A文章编号：1000-369X（2008）04-301-08EvaluationofNit
3、rogenStatusinTeaPlantsbySPAD1,2222*1*YANGYi-yang,MALi-feng,SHIYuan-zhi,RUANJian-yun,LIXing-hui（1.TeaResearchInstitute,NanjingAgricultureUniversity,Nanjing210095,China;2.KeyLaboratoryofTeaChemicalEngineering,theMinistryofAgricultureandTeaResearchInstituteofChineseAcadem
4、yofAgricultureSciences,Hangzhou310008,China）Abstract:DeterminationofnitrogenconcentrationsintissuesisanimportantdiagnosticmeansforNstatusofplants.ApotexperimentwithteaplantsappliedwithdifferentamountsofNfertilizerwascarriedouttoevaluatetheusefulnessofSPAD-502asadiagnos
5、tictool.TheresultsshowedthattheyieldofyoungshootsandNconcentrationofmatureleavesrespondedtoNapplicationrateswithapatternofaninitiallinearincreasebeforeaplatformwasreached.TherewasasignificantlypositivelinearrelationshipbetweentheSPADreadingandtotalNconcentrationofmatur
6、eleaves.ThesimilarpatternofaninitiallinearincreasefollowedbyaplatformwasobservedfortherelationshipbetweenSPADreadingandtheyield.TheseresultssuggestedthattheNstatusofteaplantsmaybecharacterizedbythemeasurementofSPADreadingofmatureleavesandtheSPADisapotentiallyusefulrapi
7、ddiagnostictoolinNfertilizationrecommendation.However,thisneedsfurthervalidationfromfieldexperiments.ThosefactorswhichmayinfluencetheprecisionofSPADmeasurement,suchasthepointoftestonaleaf,selectionofleavesfromtwig(position),weathercondition,insituorinvitroanalysis,surf
8、acecleaningandthenumberofleavesforacompositesamplewerealsoinvestigated.Keywords:CamelliasinensisL.,chlorophyllmeter,n
