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1、中国水稻科学(ChinJRiceSci),２０１６,３０(１):１－９http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５０２５１水稻小粒矮秆突变体sgd１(t)的表型分析及基因克隆汪鹏蔡跃陈韦韦马婧陈新刚唐小洁尤小满孔飞张杰燕海刚汪国湘江玲张文伟∗万建民(南京农业大学作物遗传与种质创新国家重点实验室/长江流域杂交水稻协同创新中心/农业部长江中下游粳稻生物学与遗传育种重点实验室,南京２１００９５;∗通讯联系人,EＧmail:zhangww＠njau．edu．cn)PhenotypingandGeneCloningofaSmallＧgrain
2、DwarfMutantsgd１(t)inRiceWANGPeng,CAIYue,CHENWeiＧwei,MAJing,CHENXinＧgang,TANGXiaoＧjie,YOUXiaoＧman,KONGFei,ZHANGJie,YANHaiＧgang,WANGGuoＧxiang,JIANGLing,ZHANGWenＧwei∗,WANJianＧmin(StateKeyLaboratoryofCropGeneticsandGermplasmEnhancement,NanjingAgriculturalUniversity/TheYangtzeRiverValleyHybridRiceCollab
3、orationInnovationCenter/KeyLaboratoryofBiology,GeneticsandBreedingofjaponicaRiceinMidＧlowerYangtzeRiver,MinistryofAgriculture,Nanjing２１００９５,China;∗Correspondingauthor,EＧmail:zhangww＠njaue．duc．n)WANGPeng,CAIYue,CHENWeiwei,etal．PhenotypingandgenecloningofasmallＧgraindwarfmutantsgd１(t)inrice．ChinJRice
4、Sci,２０１６,３０(１):１Ｇ９．Abstract:AsmallＧgraindwarfmutant,designatedassgd１(t),wasidentifiedfromtheTＧDNAinsertionmutantlinesofNipponbare．Themutant,geneticallystable,wascharacterizedbydwarfplant,smallgrain,darkgreenleavesandthickhusk．Scanningelectronmicroscopeanalysisoncellmorphologyofstemandhullrevealedth
5、atstemcellsinsgd１(t)failedtoformnormalcellcolumnandvascularbundles,whileepidermalcellswithirregularshapeinhullsweretightlypacked,resultinginconfusionincellarrangement,andsgd１(t)wasaGAＧsensitivedwarfmutant．Geneticanalysisshowedthatthistraitofdwarfismwascontrolledbyapairofrecessivenucleargene．Through
6、mapＧbasedcloning,thegenesgd１(t)wasmappedinanintervalof２３０kbbetweenthemarkersDF１３andDF２６ontheshortarmofchromosome９．TherewasadwarfgeneBC１２/GDD１inthisregion．Sequenceanalysisshowedthatsgd１(t)hadasingleＧbasesubstitution(GtoT)inthefourthexonofthegene,resultinginthereplacementofglycinebyvalinein１８６thconse
7、rvativeaminoacid．Keywords:rice(OryzasativaL．);smallgrainanddwarfism;genecloning;BC１２/GDD１;singleＧbasesubstitution汪鹏,蔡跃,陈韦韦,等．水稻小粒矮秆突变体sgd１(t)的表型分析及基因克隆．中国水稻科学,２０１６,３０(１):１Ｇ９．摘要:在日本晴TＧDNA突变体库中筛选得到小粒矮秆突变体sgd１
