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1、作物学报ACTAAGRONOMICASINICA2008,34(11):1958−1963http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2008.01958烟草SRAP和ISSR分子遗传连锁图谱构建11,*11213马红勃祁建民李延坤梁景霞王涛兰涛陈顺辉111陶爱芬林荔辉吴建梅123(福建农林大学作物科学学院/生命科学学院,福建福州350002
2、;福建省顺昌烟草公司,福建顺昌353200;福建省烟草公司,福建福州350003)摘要:采用烤烟品种台烟7号与白肋烟品种白肋21杂交,构建了187个单株的F2遗传作图群体,利用所筛选的68个能扩增出多态性条带的SRAP和ISSR引物,对F2作图群体进行PCR扩增和遗传连锁分析,初步构建了一张包含26个连锁群、112个(92个SRAP和20个ISSR)标记位点的烟草遗传连锁图谱。该图谱覆盖长度为1560.2cM,平均图距18.1cM。有16个标记未进入连锁群。26个连锁群包含2~20标记不等,连锁群遗传距离0~29
3、1.0cM。连锁群上有24.1%的标记出现偏分离,主要集中在LG1和LG4连锁群上,其余分散在不同连锁群。该图谱为烟草重要农艺性状的基因定位、以及分子标记辅助选择等研究奠定基础。关键词:烟草;SRAP;ISSR;遗传连锁图谱ConstructionofAMolecularGeneticLinkageMapofTobaccoBasedonSRAPandISSRMarkers11,*1121MAHong-Bo,QIJian-Min,LIYan-Kun,LIANGJing-Xia,WANGTao,LANTao,3111
4、CHENShun-Hui,TAOAi-Fen,LINLi-Hui,andWUJian-Mei12(CollegeofCroporLifescience,FujianAgricultureandForestryUniversity,Fuzhou350002,Fujian;FujianShunchangTobaccoCompany,Shun-3chang353200,Fujian;FujianTobaccoCompany,Fuzhou350003,Fujian,China)Abstract:High-densityg
5、eneticlinkagemapsareusefultoolsforstudyinggenome,phylogenyandevolution,andformappingandCloninggenesofinterest.Inthisstudy,amoleculargeneticmapwasconstructedwithF2populationconsistingof187indi-vidualsfromacrossofflue-curedtobaccocultivarTaiyan7andburleytobacco
6、cultivarBailei21byusingatotalof513SRAPprimersand42ISSRprimers.Approximately58outof513and10outof42primers,respectively,showedpolymorphisms.EachofthispolymorphicprimersproducedatleastonescorablepolymorphicDNAbandwhichwasvisibleenoughfordetectionandscoring.Thema
7、pconsistedof26linkagegroupswhichincluded112(92SRAPand10ISSR)markers,andcovered1560.2cMwithanaveragedistanceof18.1cM.Sixteenmarkerswerestillunlinked.All26linkagegroupsconsistedof2–20markersrang-inginlengthfrom0–291.0cM.Atotalof24.1%distortedmarkersdistributedi
8、nthemap,mainlyconcentratedintheLG1andLG4linkagegroups,andtheothersweremappedondiverselinkagegroupsrespectively.Thismapwillprovidethebasisforgenemappingandmarker-assistedselectionofimporta
