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1、第41卷第6期哈尔滨工业大学学报Vol41No62009年6月JOURNALOFHARBININSTITUTEOFTECHNOLOGYJun.2009生物除磷系统不同阶段性能及微生物群落分析任南琪,李楠,王秀蘅,张坤,亢涵(哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090,rnq@hit.edu.cn)摘要:为探讨工艺运行与微生物群落的关系,在厌氧/好氧SBR反应器中考察生物除磷系统的启动期、稳定期和恶化期的性能,并利用聚合酶链式反应-变性梯度凝胶电泳(P
2、CR-DGGE)技术研究该系统各阶段功能微生物群落结构特征.启动期历时26d,包括适应期和上升期两个阶段,磷的去除率从40%以下提高到80%,之后进入稳定期,磷去除率维持在80%以上.稳定期的功能微生物为Tetrasphaeraelongate,Gemmatimonasaurantiaca和Uncultured-proteobacterium.稳定运行80d后进入恶化期,磷去除率降到70%以下.恶化期和稳定期的微生物群落结构差别很大,功能微生物Uncultured-proteobacterium被淘汰,竞争菌Can
3、didatusCompetibacterphosphatiscloneSBRQ22显著增加.关键词:生物除磷;聚磷菌;性能;群落结构;PCR-DGGE中图分类号:X7031文献标识码:A文章编号:0367-6234(2009)06-0048-05PerformanceandmicrobialcommunitystructureanalysisofbiologicalphosphorusremovalsystemindifferentperiodsRENNanq,iLINan,WANGXiuheng,ZHANGKu
4、n,KANGHan(StateKeyLabofUrbanWaterResourceandEnvironment,HarbinInstituteofTechnology,Harbin150090,China,rnq@hit.edu.cn)Abstract:Inordertoinvestigatetherelationshipbetweenoperationandbiologicalcommunity,differentoperationalperiodsofbiologicalphosphorusremoval(BPR
5、)includingsetupperiod,stableperiodanddeteriorativeperiodwereanalyzedinananaerobic/aerobicsequencingbatchreactor(SBR).PCR-DGGEprotocolwasemployedforrevealingsuccessionofmicrobialcommunitystructureandchangesoffunctionalbacteriarelatedtoBPRindifferentperiods.Theset
6、upperiodlasted26days,includinglagphaseandrisingphase.Duringthesetwophases,phosphorusremovalefficiencyenhancedfromlessthan40%to80%.Aftertha,tstableperiodstartedrepresentedbystablephosphorusremovalefficiencyofmorethan80%.Tetrasphaeraelongate,Gemmatimonasaurantiac
7、aandUncultured-proteobacteriumweredominantasfunctionalmicrobesinstableperiod.Stableperiodlasted80days,andthentheBPRwentintodeteriorativeperiodwithaphosphorusremovalefficiencyoflessthan70%.Microbialcommunitystructureofstableperiodanddeteriorativeperiodvariedgre
8、atly.Uncultured-proteobacterium,whichactedaspotentialpolyphosphateaccumulatingorganisms(PAOs),waseliminatedfromthereactorwhilethemajorcompetitorCandidatusCompetibac
