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1、载体名称 上游引物（5'primer） 下游引物（3'primer）P3*FLAG-CMVCMV-30(825-854)CMV-24(1129-1152)pAc5.1/V5-His(_A,_B,_C) pAc5-5 BGHrevpAcG2T pGEX-5 notavailablepACTT7EEV T3/EBVrevpACT2GAL4ADfor pGAL4-ADrvpACT2p17110p12584pACYC184(BamHI-site) PBRforBam PBRrevBampACYCDuet-1(MCSI)ACYCD
2、uetUP1PrimerDuetDOWN1pACYCDuet-1(MCSII) DuetUp2 T7-TerminatorpAdEasy-1 notavailable notavailablepAdTrack-CMV notavailable notavailablepAS2-1GAL4Bdfor/P24990pGAL4-BDrv/P31430pB42ADpB42ADFPB42ADRpBABEpBMN_5' pBacPAK8BAC1BAC2pBAD pBAD-F pBAD-RpBAD/HisABC pBAD-5' pBB
3、R1MCS T3/M13rev T7/M13forpBD-GAL4pBD-GAL4-FPBD-GAL4-RpBI12135SNOSpBIND GAL4-Bdfor T3,EBVrevpBK-CMVM13F/T7M13R/T3pBluescriptIIKS(+/-) T3/M13rev T7/M13for（优先用M13F）pBluescriptIISK(+/-) T3/M13rev T7/M13forpBluescriptKS(+/-) T3/M13rev T7/M13forpBluescriptSK(+/-) T3/M1
4、3rev T7/M13forpBR322(BamHI-site) PBRforBam PBRrevBampBR322(EcoRI/HindIII-sites) pBRforEco pBRrevHindpBR325(BamHI-site) PBRforBam PBRrevBampBR329(BamHI-site) PBRforBam PBRrevBampBridge(MCSI) GAL4-Bdfor3'BDpBridge(MCSII) notavailable notavailablepBS-TII T3/M13rev T
5、7/M13forpBS（AMP）T7T3pBSRT3T7terpBudCE4.1(MCSI) CMV-Profor,T7 c-mycrev,EBVrevpBudCE4.1(MCSII) EF-1αFwd BGHpBudCE4.1/lacZ/CAT CMV-Profor,T7 c-mycrev,EBVrevpBV220pBV220FPBV220RpCAL-c T7/Tac-Profor T7-TerminatorpCAL-n T7/Tac-Profor T7-TerminatorpCAMBIA1301(1300)pCAMB
6、IA1301-5'(ECORI)/M13RpCAMBIA1301-3'(HindIII)pCAMBIA1304pCAMBIA1301-5'(ECORI)pCAMBIA1301-3'(HindIII)pCAMBIA2300M13R(-48)M13F(-47)/M13F(-49)pCANTB5ES1S6pCAT3-enhancerRVP3此载体无反向引物pCDFDuet-1(MCSI)ACYCDuetUP1PrimerDuetDOWN1pCDFDuet-1(MCSII)DuetUP2T7TERpcDNA1.1 CMV-Pro
7、for,T7 pCDM8-3pcDNA2.1 M13for/T7 M13rev/Tac-ProforpcDNA3 T7/CMV-Profor BGHrev/SP6pcDNA3.1(+/-) pcDNA3.1F/T7/CMV-Profor BGHrevpcDNA3.1/His(_A,_B,_C) T7/CMV-Profor BGHrevpcDNA3.1/myc-His(_A,_B,_C) T7/CMV-Profor BGHrev/c-mycrevpcDNA3.1/V5-His(_A,_B,_C) T7/CMV-Profor
8、 BGHrev/V5revpcDNA3.1/Hygro(+,-) T7/CMV-Profor BGHrev/V5revpcDNA3.1/Zeo(+.-)T7BGHrevpcDNA3.3TOPOTACMV-ProforTKPAreversepcDNA4/HisMax(_A,_B,_C) Xpressfor BGHrev
