姜黄素对过氧化氢损伤大鼠肾小管上皮细胞的保护作用

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1、中山大学硕士论文姜黄素对过氧化氢损伤大鼠肾小管上皮细胞的保护作用PartoneNRK52EcellsapoptosisinducedbyH202I．AimToinvestigatewhetherHz02caninducedNRK52Ecellsapoptosis．II．MethodsRatproximaltubularcellline(NRK一52E)wereculturedinDMEM／F12with10％FBS，100I_tg／mlstreptomycinandgentamicin(100pg／m1)at370Cand5％C02．Cellswithexpone

2、ntialphaseofgro叭hwereexposuretO100、300、500I_tmol／LH202for24h，theapoptosisofNRK52Ecellswasdetectedbypropidiumiodidestainflowcytometry(FCM)，Hochest33258stainingWasusedtOobservethemorphologicalchangesofNRK52Ecellsapoptosis．Ⅲ．StatisticalAnalysisDatawereanalyzedbySPSS13．0．Comparisonbetween

3、meanswasconductedbyANOVAanalysis．AP-valuelessthan0．05wasregardedasstatisticallysignificant．IV．Result．1．TheresultofFCMshows：Comparedwithcontrolgroup，therateofapoptosisofNRK52EcellsWasincreasedmarkedlyafterexposuretO100、300、5009mol／LH202for24h．2．ThemorphologicalchangesofNRK52Ecellsapopt

4、osisshows：thenormalcellsexhibiteduninformlydispersedchromatinandintactcellmembrane．Thecells，treatedwith100、300、5001amol／LH202for24h，appearedtypicalcharacteristicofapoptosis，includingapoptoticnuclearcondensation，andamountofNRK52EcellsaportosisWasmarkedlyincreased．8中山大学硕士论文姜黄素对过氧化氢损伤大鼠肾

5、小管上皮细胞的保护作用V．SummaryH202callinducetheNRK53ECellsapoptosis，andassumesthedosagedenpendence．ParttwoTheroleofcurcumininH202inducedI．AimapoptosisinNRK52EcellsToexploretheprotectiveeffectofcurcumin011NRK52EcellsdamageinducedbyH202，II．MethodsRatproximaltubularcellline(NRK-52E)wereculturedinD

6、MEM／F12wim10％FBS，100“g／mlstreptomycinandgentamicin(100“g／m1)at370Cand5％C02．Cellswithexponentialphaseofgrowthweredividedinto6groups：(芏)normalcontrolgroup，(室)500p．mol／LH202group，(要)201xmol／Lcurcumingroup，④40肛loⅥ。curcumingroup，⑤20岬ol／Lcurcumin+H202，⑥40岬。儿curcumin+H202．Incubatedfor24h，the

7、apoptosisofNRK52Ecellswasdetectedbypropidiumiodidestainflowcytometry(FCM)，Hochest33258stainingWasusedtOobserveThemorphologicalchangesofNRK52Ecellsapoptosis,theexpressionofBcl一2Wasmeasuredbywestemblot．KI．StatisticalAnalysisDatawereanalyzedbySPSSl3．0．Comparisonbetweenmeanswasconductedby

8、ANOVA