gbee抗肿瘤转移作用及其机制实验研究

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1、沈婷婷：GBEE抗肿瘤转移作用及其机制实验研究3Abstractobjective：Tostudytheanti—metastasiseffectofGinkgobilobaexocarpextracts(GBEE)anditsmechanismbytheestablishmentsofvarioustunqormetastasismodelsinvitroandtheC57BL／6Jmice、析thLewislungmetastasismodel；toprovidethescientificbasisfortumorpharmacology

2、revealedGBEE；andtolaythefoundationofpharmacyforthedeclarationofantineoplasticauxiliarydrug．Methods：TheestablishmentsofC57BL／6JmicewithLewislungmetastasismodelwererandomlydividedintomodelcontrol，positivecontrolandGBEE(50，100，200mg·kg卅)treatmentgroups．Moreover，thenormalcontro

3、lgroupwasbuiltwithoutinoculatingtumorcellsuspensionfluid．Themiceinmodelcontrolgroupandnormalcontrolgroupwerebothigequalvolumenormalsaline，positivecontrolgroupwereigtegafur80mg·kg-1，theGBEE(50，100，200mg·kg。11treatmentgroupswererespectivelyigGBEE50，100，and200mg·kg-1，onceadayf

4、or15d．2daysafteradministration，stripedtheturnoutandweighteditsmasstocalculatedtheinhibitoryrate．Whengetthelungtissue，themetastatictumorfocionlungsurfaceinmicewerecountedtocalculatetherateofcancerometastasisandanti．metastasisoftumoronlungsurfacebyobservedunderthedissectingmi

5、croscopeafterfixingbyBouin’Sliquid．RadioimmunoassaywasperformedtoobservethecontentsofⅣcollagen(ColIV)andhyaluronicacid(HA)inserum，immunohistochemistrymethodwasutilizedformeasuringtheexpressionsofCD44andnm23-H1oftransplantatedtumors．Invitroculture，MTTcolorimetricmethodwereem

6、ployedtodetecttheeffectsofGBEEontheproliferationofB16·F10andLLCcells，andtodetectthefunctionofGBEEonthehomogeneousadhesionbetweenB16一F10cellthemselvesandtheheterogeneousadhesionbetweenB16一F10cellsandHUVEC．AO／EBstainingwereusedtodetecttheeffectsofGBEEontheapoptosisofB16-F10ce

7、lls．ScarificmionwereemployedtoassaytheeffectsofGBEEonB16一F10cellmigration，andgelatinasezymographywereusedtodetecttheeffectsofGBEEonmatrixmetalloproteinaseactivitysecretedbyB16-FIOcells．扬州大学硕士学位论文4lksults：GBEEcarlsignificantlyinhibitthegrowthoftransplanttumoronC57BL／6JmiceLe

8、wislung，thetransplantatedtumorweightofGBEE(50，100，200mg·kg’1、treatmentgroupswere2．