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1、宁夏医科大学硕士研究生学位论文英文摘要EffectsofamiodaroneonCx43ofacutemyocardialischemiareperfusioninratsABSTRSCTObjectiveToevaluatetheprotectiveeffectofintravenousamiodaroneforacutemyocardialischemia-reperfusionandtoexploretheeffectsofamiodaroneonproteinCx43ofmyocardia
2、lgapconnections.Methods60SDrats,weighting260~300g,wererandomlydividedinto5groups(n=12):sham-operationgroup(groupS):madeacollarwithlineafterthoracotomy,butdidnottightentheligature;ischemic-reperfusiongroup(groupI/R):Tightenedtheligatureischemia30min,th
3、enrelaxedtheligature,reperfusion120min;amiodarone2.5mg/kggroup(groupA1):Intravenousinjectionbeforeischemia10min,andthencontinuedtopumptheamiodarone0.15mg/kg/minuntiltheendofreperfusion;amiodarone5.0mg/kggroup(groupA2):Intravenousinjectionbeforeischemi
4、a10min;aniodarone5.0mg/kggroup(groupA3):Intravenousinjectionbeforeischemia10min,andthencontinuedtopumptheamiodarone0.15mg/kg/minuntiltheendofreperfusion..Ratheartrate(HR).Meanarterialpressure(MAP)andtheRPP(SBP×HR)weremeasuredat10-minpre-ischemic(T0),3
5、0-minpost-ischemic(T1),30-minreperfusion(T2),60-minreperfusion(T3),120-minreperfusion(T4).Comparedarrhythmiascoreofratsineachgroupintheperiodofischemia;determinedthescrumofcTnIandCK-MBbyELISA;measuredinfarctsizebyTTCstaining;Observedchangesinmyocardia
6、ltissueusingHeidenhainstaining;observedmyocardialexpressionofCx43withimmunohistochemicalmethod.Results1.ThedifferenceofHR,MAP,RPPineachgroupwerenotstatisticallysignificant(P>0.05)inT0;ComparedwithgroupS,HR,MAP,RPPofgroupI/R,groupA1,groupA2andgroupA3we
7、resignificantlylower(P<0.05)inT1,T2,T3,T4.ComparedwiththeI/Rgroup,HRofgroupA1,groupA2andgroupA3weresignificantlyhigher(P<0.05)inIII宁夏医科大学硕士研究生学位论文英文摘要T1,T2,T3,T4,MAPofgroupA3washigherthangroupI/RonlyinT2,T3(P<0.05),therewerenosignificantdifferencebetw
8、eentherestofthegroups(P>0.05).ComparedwithT0,HR,MAPofgroupI/R,groupA1,groupA2andgroupA3haddifferentdegreesofreduction(P<0.05)inT1,T2,T3,T4,butHR,MAPofgroupA3showedagradualrecoverytrendineachtimepointofreperfusion.2.ComparedwithgroupS,arrhythmi
