洒金桃叶珊瑚叶中桃叶珊瑚苷的提取纯化与质量标准的的研究

《洒金桃叶珊瑚叶中桃叶珊瑚苷的提取纯化与质量标准的的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、西南交通大学硕士研究生学位论文第1I页=m==；m；m==；ii；=；===；；；==i=====；ii=i．i=；i=；．．ii．．‘．—．．—．——．．．‘．．．—．．．．．．．．．．．—．．———．．．．．．．．．．—．．．．．—．．．．—．．．．．．．—．．—．——————．．————————————————————————————————————ImAbstractObjective：InvestigatethetechniqueofextractingandpurifyingaucubinfromAucubajapon

2、icaVariegata．Studyonthequalitystandardofaucubin，anddrawupqualitystandarddraft．Evaluatethestabilityofaucubin．Method：ExtractaucubinfromAucubajaponicaVariegatathroughLoopscavenging，measurecontentofaucubinbyHPLC．AdoptmacroporousresinS一8andSilicagelseparateaucubin，preferen

3、cethebestcondition．Purifyaucubinthroughcrystallizationmethod，andthenmakestructuralidentification．Evaluatequalityofaucubinbywayofdescribingcharacter,measuringphysicochemicalconstants，establishingdifferentialandcheckedmethod，alsotheWaytomeasurementoftheaucubincontent．Ad

4、opteffectfactortestevaluatestabilityofaucubin．Result：附(ingaucubinasindexes．thebestresultofmacroporousresinS一8iSasfollows：sampleconcentration2．76mg·mL～，feedrate2BV／h，samplecapacity9．56mg／g，eluantl5％ethanolwater,elutionflowrate2BV／h．totalquantityofeluant3BV．Thebestcondi

5、tionofsilicagelcolumn：eluantCH30H—CHCl3(1：4，1：3)，samplecapacity35mg／ml，elutionvolume3BV．elutionflowrate2BV／h．Accordingtothemethod，thecontentandtherecoveryrateof79．19％and83．38％．Aftercrystallizationprocess．elaborationproductwouldbegot．Investigatephysicochemicalproperty,

6、discrimination，examination，assayandstabilityofaucubin．drawupqualitystandarddraft．Aucubiniswhiteoralmostwhitecrystallinepowder,odorless，water-soluble．Itiswater-solubleandmethanol—soluble，ethanolsparinglysoluble，butinorganicsolvent1ikeCHCl3，petroleumetheriSundissolved．I

7、tiSeasytoabsorbmoist．Specificrotationofaucubinis．117O．QualitativeidentificationiSmadebyTLC，HPLC．andIRandSOon．ThetestofreleaseisaccordingtoChinaPharmacopeia(2010edition)．andprovidethelowestcontentsofimpurity．HPLCcanbeappliedtodeterminationofaucubin．Theresultshowsthatth

8、emethodiSsatisfactory,well．repeatable．TheresultofstabilitytestindicatedthataucubiniSunstablewhenitcomestohigh-temperatureand