猕猴桃品种间的遗传多样性分析

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1、四川大学硕士学位论文猕猴桃品种问的遗传多样性分析AnalysisofgeneticdiversityinAcitinidiavarietiesMajorinGeneticsGraduatestudent"ZouYou№九lctor：YangZhirongABSTRAC’I’KiwifruitisallimmemorialplantwhoseleaffossilwasanalyzedtohavebeensubsistedformoDethan26millionyears．Sixty·sixkindsofspeciesexistinallOVa"thew

2、orld，sixty—twoofwhichweredistributedinChina．Chinaistheorighalhabitatof鼬wifruit．wherethereareabundantsources．IthasbeenliterallyhistoricizedformodethantwothousandyearsinChin,-LThenutritivevalueandofficinalhygienicallyvaluesarehi曲inKiwifruit．VitaminCisespeciallyhigh，whichisknown罄t

3、hekingoffruitageorthekingofVitaminC．Takenasarisingfruit．Kiwifmitisnowthedemandedmoreandmoreincreasingly．Distinctivevarietiesfrommanycountriesareemulativelyextendedtooccupythesailingmarket．Sothebreedingofdistinctivenewvarietiesbecamethekeyforcompetition．Schleppingexcellentgenes，

4、germplasmisthematterbasisofbreedingnewvarietyandisevenmorethesourceofdiscoveringandutilizinggeneresources．Theresearchofgeneticdiversityforgermplasmisavitalsegmentforgermevaluation,whichCanprovidemuchimportantgeneticinformationforresourc宅protectionandsoundutilization．Inthisstudy

5、,randomamplifiedpolymorphicDNA(RAPD)andintersimplesequencerepeats(ISSRlanalysisWel"eusedtodetectthegeneticdifferentiationofKiwifruitvarietiesfromtheCentreofKiwifruitResearchandDevelopment，SichuanNaturalResourcesResearchInstitution．tOestablishallefficientRAPDandISSR-basedgenetic

6、diversitydetectionsystem，6四川大学硕士学位论文猕猴桃品种问的遗传多样性分析Firstly,RAPDanalysiswasusedtodetectthegeneticdifferentiationof1Fdwifruitvarieties．DNAofvarietieswasamplified埘tll15randomprimers．ExperimentalconditionsforRAPDsystemwereexploredandimproved．Geneticdistanceindexesamongvarietieswerec

7、alculatedbyNei，andthephylogenetictreeWasconstructedbyUPGMAmethod．Secondly,ISSRanalysiswasitsedtodetectthegeneticdifferentiationof14Kiwifruitvarietiesincludingfirstpart,gettingoptimizedISSRsystemexperimentalconditionandvalidating埘tlltheconsistencyofRAPDclassified．Tb￡resultsshow：

8、I，BothRAPDandISSRcanbeusefulmarkersfortheanalysisofgen