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1、人造血干细胞扩增的研究进展论文关键词：干细胞人造血progressonStudyofTechniquesforExpansionofHumanHematopoieticStemCells造血干细胞（llematopoieticstemcells,HSC）移植可能使遗传血液病、免疫缺陷病及恶性肿瘤等完全治愈，由于HSC移植存在以下不足而受限制：⑴为获得足够量移植用HSC，必须进行大规模的骨髓抽吸或餐周血分离；⑵获得的HSC量有限；⑶HSC输注后产生的成熟细胞的生物动力欠佳，移植后1-3周内并无直接治疗疗效。移植前
2、HSC培养扩增，可解决这些难题。当前主要有两种：⑴细胞因子支持下筛选CD34+细胞的体外扩增；⑵基质支持下的灌注培养。下面就HSC培养的发展、HSC扩增方法以及展望等作一综述。1早期HSC培养及启示1半固体培养体系1966年，Bradley等1介绍了一种新的半固体培养方法（即集落形成法）用以培养骨髓。此法利用胶体凝胶作支托，没有细胞微环境，只能维持造血祖细胞在1-2周内形成细胞集落.freell-1的浆灌注为0.1ml·kg-1·min-1，相当于细胞密度为106·ml-1，含20%血清的培养介质，每天全部更换
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