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1、Abstractincreased,andtheinhibitionhasthetimeandtheconcentrationdependence,statisticallysignificantdifference(P<0.05);2．Flowcytometryresultsshowed:(1)3,6,12mumol/LsunitibrolerespectivelyinKYSE70cellsandKYSE450after48h,apoptosisrate(33.20+0.91)%,(46.00+1.35)%,(69.00+1.56)%a
2、nd(35.00+0.21)%,(47.00+0.98)%,(74.30+1.28)%,whilethecontrolgroup,apoptosisratewas(1.30+0.26)%and(1.50+0.34)%.Afterstatisticalanalysisshowedthatwiththeincreaseofconcentrationofsunitinibcellapoptosisratealsograduallyrise,theconcentrationdependenceofremarkable,andcomparedwit
3、htheblankcontrolgroup,thedifferencestatisticallysignificant(P<0.5).Thedifferencebetweentwogroup(P=0.053)。3．Three-dimensionalculturesystemtoobservetheformationoftubularstructuresineachexperimentalgroupnumber:afterabout12hoursKYSE70andKYSE450cellswillformedtypicalvascularne
4、tworkstructure,asingleloopormultipleringsconnectedtogrid.Sunitinibeffects24hoursafterthetwokindsofcellsformingabilityweresignificantlyinhibited,annularstructurefracture,isnotcompletelyclosedcircularandlinearstructuremore,themoreagreatnumberofstructureandconcentrationsigni
5、ficantlyless,statisticallysignificantdifference(P<0.5);Butnodifferencebetweenthetwodifferentgroupsofcells(P=0.054).4．RT-PCRassaytheVM-relatedmoleculesmRNAexpressiongroup:after48hourssunitinibeffecttheexpressionofc-met,mek,VE-cad,MMP-2mRNAdecreasedobviously,thedifferencest
6、atisticallysignificant(P<0.05),andc-metwithconsistentmekloss,VE-cardinconsistentwithMMP-2drops;Andnostatisticaldifferencebetweentwogroupsofcellscontrast(P=0.06).Conclusion1．Sunitinibthroughmultiplepathwayseffectivelyrestrainhighandlowdifferentiationofesophagealsquamouscan
7、cercellsandtheexpressionofVE-Cad,c-met,bycuttingMMP-2expressionandinhibitingangiogenesis,theformationofmimicryandotherways,andinhibitionofcellproliferationandpromoteapoptosiseffectintofullplay.VIII万方数据Abstract2．Multitargetstargetedtherapyinesophagealsquamouscellcarcinomas
8、haveafurtherresearchprospectsincomprehensivetreatment.KeyWords：Esophagealneoplasms;sunitinib;Vas
