不同龋敏感者口腔变形链球菌基因型与产酸性之间的关系.doc

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1、不同龋敏感者口腔变形链球菌基因型与产酸性之间的关系作者：李伟，贺新兰，胡红梅，张铮辉，王蔚，周鹏，汤洪，刘兴容【摘要】　　目的：研究不同龋敏感者口腔变形链球菌基因型与产酸性的关系.方法：①随机选取50例24～32岁的高龋、中龋及无龋者，取牙面菌斑样本接种于MS培养基上，每人随机挑取2～5株临床分离株，提取细菌染色体DNA，采用AP-PCR法对不同龋敏感者口腔变形链球菌临床分离株进行基因分型.②以pH0.5为间隔配制pH4.0～7.0系列的，含50mg/L葡萄糖的MS液体培养基，对不同基因型口腔变形链球菌临床分离株进行厌氧培养48h，用精密

2、pH计检测培养基上清液的pH值.结果：①14例高龋个体中有3例为1种基因型，5例有2种基因型，6例有3种基因型；9例中龋个体中有4例为一种基因型，3例有2种基因型，2例有3种基因型；13例无龋个体中有12例为1种基因型，1例有2种基因型.②不同基因型的变形链球菌培养基上清液的pH值进行统计学分析，发现基因型越多的细菌其产酸能力越强.结论：①龋敏感越高的个体其口腔变形链球菌临床分离株基因型越多；②不同基因型的变形链球菌pH值的检测，基因型越多的细菌其产酸能力越强.【关键词】龋敏感者随机引物聚合酶链法基因型链球菌变异　　Association

3、betweengenotypeandacidogenicityofStreptococcusmutansinsubjectswithdifferentcariessusceptibility　　【Abstract】AIM:TostudythegenotypeofStreptococcusmutansisolatedfromdifferentcaries-susceptiblesubjectsandtoanalysetheassociationofthegenotypewithacidogenicityofStreptococcusisol

4、ates.METHODS:Plaquesampleswereobtainedfrom50childrenwhowere24to32yearsoldandoriginallyplatedontoMitis-Salivarius-Bacitracinagar．ChromosomalDNAwasextractedfromtheisolatesobtainedfromsamplesbyDNAkit.ThengenotypingofStreptococcusmutanswascarriedoutbyarbitrarilyprimedpolymera

5、sechainreaction(AP-PCR).StreptococcusmutanswereinoculatedinMSmediacontaining5mg/LglucoseandwithdifferentpHvalues(4.0～7.0),andgrewinanaerobiccondition（80mL/LN2,100mL/LH2,100mL/LCO2）at37℃for48h.ThefinalpHvaluesofthesolutionsweremeasured,andthencompared.RESULTS：IsolatesofStr

6、eptococcusmutanswereobtainedfrom36subjects(total50subjects).101Streptococcusmutansstrainswereobtainedand31genotypeswerefound.Morethanonegenotypeisolateswerecolonizedinthesameindividualof14high-cariessubjects,6possessedthreegenotypes,5possessedtwo6genotypes，and3hadonegenot

7、ype;of9middle-cariessubjects,2possessedthreegenotypes,3possessedtwogenotypes，and4hadonegenotype;of13cariesfreesubjects，12hadonegenotype,1possessedtwogenotypes.StatisticalanalysisofthefinalpHvaluesofculturesupernatantsrevealedthattheisolateswithmoregenotypespresentedthestr

8、ongerabilityofacidogenicity.CONCLUSION：AP-PCRtechnologycanobtaingeneticmapofStreptococcusmutans.