毕业论文--催产素预处理对胎鼠海马脑片缺氧无糖损伤后otr、gabaarβ表达的影响

《毕业论文--催产素预处理对胎鼠海马脑片缺氧无糖损伤后otr、gabaarβ表达的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、催产素预处理对胎鼠海马脑片缺氧无糖损伤后OTR、GABAARβ1表达的影响【摘要】目的：研究催产素(OT)预处理对胎鼠海马脑片缺氧无糖损伤后催产素受体(OTR)、γ氨基丁酸A受体β1亚基(GABAARβ1)表达的影响，探讨OT神经保护的作用机制。方法：采用胎龄20dSD大鼠海马脑片，实验组经含10-6mol·L-1OT人工脑脊液(ACSF)孵育预处理后行缺氧无糖损伤(OGD)，对照组经正常ACSF孵育后直接行OGD，各15min，SP免疫组化法半定量测定海马CA1区OTR、GABAARβ1表达变化，电镜观察神经元损伤情况。结果：实验组海马CA1区OTR、GABA

2、ARβ1表达均显著高于对照组(t=2.118、P=0.048;t=2.272、P=0.036)。对照组大量神经元受损,核固缩或碎裂，部分核膜溶解，实验组细胞核、粗面内质网、线粒体均较对照组有所改善,核膜较光滑,胞浆肿胀较对照组减轻。结论：OT上调胎鼠海马OTR、GABAARβ1表达，减少神经元变性坏死，增强大脑耐受缺氧损伤的能力，对胎儿中枢神经系统起保护作用。【关键词】催产素;海马;缺氧无糖损伤;催产素受体;γ氨基丁酸A受体β1亚基　　[Abstract]Objective：Tostudytheeffectsofoxytocin(OT)onchangesofox

3、ytocinreceptor(OTR),12gammaamimobutyricacidreceptorAβ1subunit(GABAARβ1)expressionsinfetusrathippocampusinducedbyoxygenandglucosedeprivation(OGD),andtoinvestigatethemechanismofneuroprotectiveeffectsofOT.Methods：Gestationalage20daysSDrathippocampusslicesintheexperimentalgroupunderwentp

4、retreatmentincubationbycontaining10-6mol·L-1OTartificialcerebrospinalfluid(ACSF),thecontrolgroupwereincubatedwithnormalACSF.Oxygenandglucosedeprivation(OGD)wasdonefor15min,respectively.SPimmunohistochemistryandsemiquantitativetechniqueweretakentodeterminehippocampusCA1areaOTR,GABAARβ1

5、expressions,andobserveneuronaldamageinelectronmicroscopy.Results:TheexpressionsofOTR,GABAARβ1inCA1areaofhippocampusweresignificantlyhigherintheexperimentalqroup(t=2.118,P=0.048;t=2.272,P=0.036).Alargenumberofdamagedneurons,nuclearcondensationorfragmentation,someofthenuclearmembranedis

6、solvedinthecontrolgroup;whilenuclei,roughendoplasmicreticulum,mitochondriawereimproved,nuclearmembranewassmooth,cytoplasmicswellingwereeasedintheexperimentalgroup.Conclusion:OTcanincreasehippocampalOTR,GABAARβ1expressions,decrease12neuronaldegenerationandnecrosis,enhancethecapacityoft

7、hebraintolerancetohypoxia,andplaytheprotectiveeffectsonfetalnervoussystem.　　[Keywords]oxytocin;hippocampus;oxygenandglucosedeprivation;oxytocinreceptor;gammaamimobutyricacidreceptorAβ1subunit　　新生儿缺氧缺血性脑病(hypoxicischemicencephalopathy,HIE)是围产期缺氧、窒息导致脑的缺氧缺血性损害，临床出现一系列中枢神经系统异常表现的疾病，是引起

8、新生儿死亡