不同龋敏感者口腔变形链球菌基因型与产酸性之间的关系

《不同龋敏感者口腔变形链球菌基因型与产酸性之间的关系》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、不同龋敏感者口腔变形链球菌基因型与产酸性之间的关系作者：李伟，贺新兰，胡红梅，张铮辉，王蔚，周鹏，汤洪，刘兴容【摘要】　　目的：研究不同龋敏感者口腔变形链球菌基因型与产酸性的关系.方法：①随机选取50例24～32岁的高龋、中龋及无龋者，取牙面菌斑样本接种于MS培养基上，每人随机挑取2～5株临床分离株，提取细菌染色体DNA，采用AP-PCR法对不同龋敏感者口腔变形链球菌临床分离株进行基因分型.②以pH0.5为间隔配制pH4.0～7.0系列的，含50mg/L葡萄糖的MS液体培养基，对不同基因型口腔变形链球菌临床分离株进行厌氧培养48h，用精密pH计

2、检测培养基上清液的pH值.结果：①14例高龋个体中有3例为1种基因型，5例有2种基因型，6例有3种基因型；9例中龋个体中有4例为一种基因型，3例有2种基因型，2例有3种基因型；13例无龋个体中有12例为1种基因型，1例有2种基因型.②不同基因型的变形链球菌培养基上清液的pH值进行统计学分析，发现基因型越多的细菌其产酸能力越强.结论：①龋敏感越高的个体其口腔变形链球菌临床分离株基因型越多；②不同基因型的变形链球菌pH值的检测，基因型越多的细菌其产酸能力越强.12【关键词】龋敏感者随机引物聚合酶链法基因型链球菌变异　　Associationbetw

3、eengenotypeandacidogenicityofStreptococcusmutansinsubjectswithdifferentcariessusceptibility　　【Abstract】AIM:TostudythegenotypeofStreptococcusmutansisolatedfromdifferentcaries-susceptiblesubjectsandtoanalysetheassociationofthegenotypewithacidogenicityofStreptococcusisolates.ME

4、THODS:Plaquesampleswereobtainedfrom50childrenwhowere24to32yearsoldandoriginallyplatedontoMitis-Salivarius-Bacitracinagar．ChromosomalDNAwasextractedfromtheisolatesobtainedfromsamplesbyDNAkit.ThengenotypingofStreptococcusmutanswascarriedoutbyarbitrarilyprimedpolymerasechainrea

5、ction(AP-PCR).StreptococcusmutanswereinoculatedinMSmediacontaining5mg/LglucoseandwithdifferentpHvalues(4.0～7.0),andgrewinanaerobiccondition（80mL/LN2,100mL/LH2,100mL/LCO2）at37℃for48h.ThefinalpHvaluesofthesolutionsweremeasured,andthencompared.RESULTS：IsolatesofStreptococcusmut

6、answereobtainedfrom36subjects(total50subjects).101Streptococcusmutansstrainswereobtainedand1231genotypeswerefound.Morethanonegenotypeisolateswerecolonizedinthesameindividualof14high-cariessubjects,6possessedthreegenotypes,5possessedtwogenotypes，and3hadonegenotype;of9middle-c

7、ariessubjects,2possessedthreegenotypes,3possessedtwogenotypes，and4hadonegenotype;of13cariesfreesubjects，12hadonegenotype,1possessedtwogenotypes.StatisticalanalysisofthefinalpHvaluesofculturesupernatantsrevealedthattheisolateswithmoregenotypespresentedthestrongerabilityofacid

8、ogenicity.CONCLUSION：AP-PCRtechnologycanobtaingeneticmapofStreptococcusmuta