心麻痹液在缺血预处理产生心肌保护效应中的作用研究

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1、心麻痹液在缺血预处理产生心肌保护效应中的作用研究【关键词】缺血预处理NiuHao，ZhaoJinxian，ZhuBinDepartmentofinternalmedicine，ZhongtangPeople’sHospital，Dongguan523220.【Abstract】ObjectiveToinvestigatethefunctionofcardioplegiainIPC-inducedcardioprotectionthroughcomparingtheeffectsofischemicpreconditioning（IPC）andIP

2、Cfollowedbycardioplegiaonimmaturerabbitheartsgloballysufferedischemia-reperfusioninjury.MethodsNewZealandrabbitsaged14-21daysweighing220-280gwereused.Theanimalswereanesthetizedandheparinized.ChestwasopenedandheartwasquicklyremovedandaortawasconnectedtoLangendorffwithin30safte

3、rexcision.TheheartswereperfusedwithKrebs-Henseleitbufferbalancedwithgasmixture（O2:CO2=95%:5%）at60cmH2O（perfusionpressure）.IPCconsistedof5minglobalisˉchemiaplus10minreperfusion.Thirtytwoimmaturerabbitheartswererandomlydividedintofourgroups:CONⅠ（n=8），wasonlymadegloballyischemic

4、bywithholdingperfusionfor30minfollowedby40minreperfusion;IPC（n=8），IPCand30minnoreperfusionfollowedby40minreperfusion;CONⅡ（n=8），Acardiacarrestwasinducedwith4℃StThomasⅡcardioplegicsolutionanda45minnor　eperfusionfollowedby40minreperfusion;IPC+St.Ⅱ（n=8），IPC，4℃StThomasⅡcardioplegi

5、aand30minnoreperfusionfollowedby40minreperfusion.Coronaryflow（CF），HR，leftventricledevelopedpressure（LVDP），and±dp/dtmaxweremonitoredatequilibration（baselinevalue）andthe5，10，20，30and40minafterreperfusion.Thevaluesgotafterreperfusionwereexpressedaspercentageoftheirbaselinevalˉue

6、.Myocardialenzymeinthecoronaryeffluent，andmyocardialenergeticmetabolismwerealsodetermined.ResultsThefourhemodynamicsindexeshavenotsignificantdifferencebetweentheIPCgroupandtheCONⅠ.IttooktheIPCgroupmuchlongertimetocardiacischemia-beating（16.23±1.74minversus13.50±1.82minincontr

7、olsP<0.01）.TheCK-MBleakageinIPCgroupwasincreased，althoughtheresultswerenotsignificantlydifferentfromcontrol.ThemyocardiallevelsofATPinpreconditioninggroupattheendofthereperfusionwassignificantlydecreased.YetinIPC+St.ⅡgrouptherecoveryofCF，HR，LVDPand±dp/dtmaxwassignificantlyimp

8、rovedcomparedwithCONⅡandlessCK-MBleakedoutandaswellaspreservedmyocar