天麻总提物对小鼠学习记忆能力的影响-论文.pdf

《天麻总提物对小鼠学习记忆能力的影响-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、·50·ChineseJournaIoflnformationOl3TCMSep．2014VoI．21No．9天麻总提物对小鼠学习记忆能力的影响韩春妮，何芳雁，田野，段小花云南中医学院，云南昆明650000摘要：目的观察天麻总提物对小鼠学习记忆能力的获得、巩固、再现功能的影响，为，临床研发新药提供依据。方法昆明种雄性小鼠随机分为正常组、模型组、对照组、天麻总提物组，各给药组给予相应药物灌胃，灌胃容积为0．2mL／lOg，正常组与模型组给予等容积蒸馏水灌胃，连续16d。于给药l1d后复制东莨菪碱、氯霉素、乙醇致小鼠记忆获得、巩固、再现功能障碍

2、模型，采用Morris水迷宫进行定位航行实验及空间探索实验，测定小鼠逃避潜伏期和空间搜索距离，以及原平台象限时间百分比和距离百分比。结果天麻总提物能明显缩短模型小鼠逃避潜伏期及搜索距离(P

3、2014)09—0050—03EfleetsofExtractivefromGastrodiaeR血izomaonLearning—MemoryFunctioninMiceHANChun-ni，HEFang—yan，TIANYe，DUANXiao-hua(YunnanUniversityofTraditionalChineseMedicine,Kunming650000,China)Abstract：0bjectiveToevaluatetheeffectsofextractivefromGastrodiaeRhizomaonacquisi

4、tion，consolidationandretrievaloflearning—memoryfunctioninmice：Toprovidesomereferenceforclinicalresearchanddevelopmentofnewdrugs．MethodsMaleKunmingmicewererandomlydividedintocontrolgroup，modelgroup，positivecontrolgroupandGastrodiaextractivegroup．PositivecontrolgroupandGastr

5、odiaextractivegroupweregivengavagebyusingrelevantmedicine0．2mL／10g，andthecontrolgroupandmodelgroupweregivengavagewiththesalTleamountofdistilledwaterfor16days．Afterreceivinggavageforcontinuous11days．memoryacquisitionbarriermodelwasinducedbyscopolamine；memoryconsolidationbar

6、riermodelwasinducedbychloromycetin：memoryretrievalbarriermodelwasinducedbyEtOH．Thelearning—memoryfunctionwasreviewedbyescapelatencyandspatialsearchdistance．ThequadrantanddistancesearchtimepercentagewasdetectedthroughdirectionalnavigationtestandspatialprobetestinMorriswater

7、maze．ResultsExtractivefromGastrodiaRhizomashortenedthetimeforacquisition，consolidationandretrievaloflearningmemoryaboutescapelatencyandspatialsearchdistance(P

8、hizoacaneffectivelyimprovetheacquisition，consolidationandretrievaloflearning—memoryfuncti