资源描述：
《冠状病毒Coronavirusl论文-1978 Determination of coronavirus 229E antibody by an immune-adherence hemagglutination method.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、JournalofMedicalVirology2:215-223(1978)DeterminationofCoronavirus229EAntibodybyanImmuneAdherenceHemaggIutinationMethodGiuseppeGerna,GiorgioAchilli,ErcoleCattaneo,andPaoloCeredaVirologyLaboratory,InstituteofInfectiousDiseases,UniversityofPavia,Pavia,Ita
2、lyAnimmune-adherencehemagglutination(IAHA)methodforcoronavirus229Eantibodydeterminationhasbeendevelopedbothfordiagnosisofrecentinfectionsandfordetectionoflong-pastinfections.Resultshavebeencom-paredwiththoseobtainedbycomplementfixation(CF),neutralizati
3、on(Nt),andindirecthemagglutination(IHA)tests.TheIAHAmethodhasbeenshowntobeassensitiveastheCF,Nt,andIHAtestsindetectingcasesofacute229Einfection.However,inaseroepidemiologicalsurveyof343healthypeopleofallages,IAIIAdetected229Eantibodyin254individuals(74
4、.0%),Ntin166(48.3%),IHAin89(25.Y%),andCFin30(8.7%).Astudyoftheprevalenceofcoronavirus229EIAHAantibodyinthedifferentagegroupshasshownthatduringtheseconddecadeoflifenearly100%ofthepopulationacquirethistypeofantibody,whereasonly50%arepositiveattheendofthe
5、firstdecade.Intheolderagegroups,thehighfrequencyofCFantibody(“marker”ofrecentinfection)indirectlyconfirmsthehighrateof229EreinfectionsandthenonprotectivenatureofIAHAantibody.CFtiter21:8in90%ofcasescorrespondedtoIAIIAtiters21:64.However,serawithIAHAtite
6、rsof21:128wereoftenCF-negative.Recent2298in-fections(orreinfections),asdeterminedbythepresenceofCFantibody,weremorefrequentinApril-MaythaninOctober-November.Threecasesofacuteinfectionshowing229Eseroconversion(twoadultsandonechild)wereobservedduringthew
7、inter-springseason.IAHAappearstobethetestofchoiceforseroepidemiologicalsurveys.Keywords:coronavirus2298,immune-adherencehemagglutination,viralrespiratoryinfectionsINTRODUCTIONEpidemiologicalsurveyscarriedoutintheUnitedStates[1-31,England[4],andBrazil[5
8、]indicatethatcoronavirus229Einfectionsareworldwide.Studiesofprevalenceofcoronavirus229Eantibodyhavebeenperformedusingcomplementfixation(CF)[6],neutralization(Nt)[l],andindirecthemagglutinationtests(IHA)[7].CFantibodyReceivedforpublicati
