美赛论文模板(强烈推荐).doc

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1、Team#9530Page2of6TitileSummaryDuringcelldivision,mitoticspindlesareassembledbymicrotubule-basedmotorproteins1,2.Thebipolarorganizationofspindlesisessentialforpropersegregationofchromosomes,andrequiresplus-end-directedhomotetramericmotorproteinsofthewidelyconservedkinesin-5(BimC)family3.Hypoth

2、esesforbipolarspindleformationincludethe'push−pullmitoticmuscle'model,inwhichkinesin-5andopposingmotorproteinsactbetweenoverlappingmicrotubules2,4,5.However,thepreciserolesofkinesin-5duringthisprocessareunknown.Hereweshowthatthevertebratekinesin-5Eg5drivestheslidingofmicrotubulesdependingonth

3、eirrelativeorientation.WefoundincontrolledinvitroassaysthatEg5hastheremarkablecapabilityofsimultaneouslymovingat20nms-1towardstheplus-endsofeachofthetwomicrotubulesitcrosslinks.Foranti-parallelmicrotubules,thisresultsinrelativeslidingat40nms-1,comparabletospindlepoleseparationratesinvivo6.Fur

4、thermore,wefoundthatEg5cantethermicrotubuleplus-ends,suggestinganadditionalmicrotubule-bindingmodeforEg5.Ourresultsdemonstratehowmembersofthekinesin-5familyarelikelytofunctioninmitosis,pushingapartinterpolarmicrotubulesaswellasrecruitingmicrotubulesintobundlesthataresubsequentlypolarizedbyrel

5、ativesliding.Weanticipateourassaytobeastartingpointformoresophisticatedinvitromodelsofmitoticspindles.Forexample,theindividualandcombinedactionofmultiplemitoticmotorscouldbetested,includingminus-end-directedmotorsopposingEg5motility.Furthermore,Eg5inhibitionisamajortargetofanti-cancerdrugdeve

6、lopment,andawell-definedandquantitativeassayformotorfunctionwillberelevantforsuchdevelopmentsTeam#9530Page2of6ContentTitile1Summary11Introduction11.1RestatementoftheProblem11.2Background11.1.1CommonSolvingTechnique11.1.2PreviousWorks11.3Example12AnalysisoftheProblem12.1OutlineoftheApproach12.

7、2BasicAssumptions12.3DefinitionsandKeyTerms13CalculatingandSimplifyingtheModel14TheModelResults15ValidatingtheModel16StrengthsandWeaknesses16.1Strengths16.2Weaknesses17FoodforThought18Conclusion1References1Appendices1AppendixASourceCode1Appen