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1、ARTICLEINPRESSDevelopmental&ComparativeImmunologyDevelopmentalandComparativeImmunology30(2006)1203–1215www.elsevier.com/locate/devcompimmCloningandcharacterizationofthedromedary(Camelusdromedarius)neonatalFcreceptor(drFcRn)a,aaaImreKacskovics,Bala´zsMayer,Zs
2、uzsannaKis,La´szlo´V.Frenyo´,bcbYaofengZhao,SergeMuyldermans,LennartHammarstro¨maDepartmentofPhysiologyandBiochemistry,FacultyofVeterinaryScience,SzentIstva´nUniversity,Istvanutca2.H-1078,Budapest,HungarybClinicalImmunology(F79),KarolinskaUniversityHospital,H
3、uddinge,SE-14186Stockholm,SwedencLaboratoryofCellular&MolecularImmunology,DepartmentofMolecular&CellularInteractions,VlaamsInteruniversitairInstituutvoorBiotechnologie,VrijeUniversiteitBrussel,Pleinlaan2,1050Brussel,BelgiumReceived10January2006;receivedinrevi
4、sedform23February2006;accepted25February2006Availableonline24March2006AbstractThefulllengthcDNAofthedromedaryneonatalFcreceptor(drFcRn)achainwasisolatedandfoundthatitissimilartotheneonatalFcreceptor(FcRn)ofotherspecieswithahighoverallsimilaritytoruminantFcRna
5、chains.ThedrFcRn/Fccontactresiduesarehighlyconservedandpredictedtobindbothconventional(IgG1)andheavychain(IgG2a,IgG3)antibodies.Usingimmunohistochemistry,wedetecteditsexpressioninthehepatocytesandinepithelialcellsofportalbileductuliandalsointhemammaryglandaci
6、niandducti.Remarkably,Ser313,thatwasidentifiedtobecrucialforapicaltobasolateraltranscytosis,issubstitutedinthedrFcRnachain.ThefulllengthofthedogandorangutanFcRnachainswasalsoidentifiedfromdatabases.Analyzingthephylogeneticrelatednessofthisgenewefoundthatdromeda
7、ryclusteredtogetherwithartiodactyls,dogislocatedbetweenartiodactylsandprimates,wheretheorangutanwasbranched,reflectingtheacceptedevolutionaryrelationships.r2006ElsevierLtd.Allrightsreserved.Keywords:Antibodies;Fcreceptors;Dromedary;Mammarygland;Liver1.Introduc
8、tionwithintheArtiodactylswasaccompaniedbytheappearanceofadditionalclassesoffunctionalAlthoughthecamelisaruminant(orapseudo-antibodies,composedsolelyofheavychains[1].ruminant),itsimmunoglo
