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ID:36515292
大小:689.62 KB
页数:76页
时间:2019-05-11
《灵杞黄斑颗粒含药血清对兔视网膜色素上皮细胞氧化损伤的保护作用及机制研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、上海交通大学硕士学位论文灵杞黄斑颗粒含药血清对兔视网膜色素上皮细胞氧化损伤的保护作用及机制研究姓名:李彩红申请学位级别:硕士专业:眼科学指导教师:邱庆华20080501±0.74%,ML和MH组的凋亡率比M组明显减少。TUNEL检测结果:NC组中未见明显凋亡细胞,M组中可见大量胞核黄色着染的凋亡细胞,ML组中散见胞核黄色着染的凋亡细胞,数量明显少于M组,MH组凋亡细胞进一步减少。MDA含量及SOD活性测定:与NC组比较,M组RPE细胞MDA含量明显增高,SOD活性明显下降;ML组MDA水平增高幅度较M组减小,MH组更加减小,ML组和MH组SOD水平与M组相比均明显升高。Ca
2、spase-3和Bcl-XL表达检测:M组Caspase-3蛋白表达上调,显著高于ML和MH组;而Bcl-XL蛋白表达下调,显著低于ML和MH组;结论:灵杞黄斑颗粒含药血清对兔RPE细胞氧化损伤具有一定的保护作用,且呈剂量依赖性。推测其可能通过清除自由基,下调Caspase-3并上调Bcl-XL的表达以发挥保护作用。关键词:灵杞黄斑颗粒,老年性黄斑变性,视网膜色素上皮细胞,氧化应激,血清药理学,凋亡ProtectiveeffectandMechanismof“LingqiHuangbanGranula”onoxidativestress-inducedinjuryinrab
3、bitretinalpigmentepithelialcellsAbstractObjective:ToobservetheprotectiveeffectandMechanismofdrug-containedserumof“LingqiHuangbanGranula”(LQHB)onrabbitretinalpigmentepithelial(RPE)cellsofoxidativestressinducedinjuryinvitro.Methods:TheoxidativestressmodelofculturedrabbitRPEcellswasinducedwit
4、hhydrogenperoxide(500µmol/L)anddifferentconcentrationsdrug-containedserumofLQHBwereadministered.RPEcellswererandomizedintonormalcontrolgroup(NCgroup),modelgroup(Mgroup),model+normalserumgroup(MSgroup),model+low-doseLQHBgroup(MLgroup)andmodel+high-doseLQHBgroup(MHgroup).Thelevelofcellprolif
5、erationwasmeasuredbytheMTTmethod,TUNELandFlowCytometer(FCM)methodswereusedtomeasuretheapoptosisofculturedrabbitRPEcells.Thelevelofmalondialdehyde(MDA)andtheactivityoftotalsuperoxidedismutase(T-SOD)inthecellweredeterminedtoreflecttheoxidant-antioxidantstatus.TheproteinexpressionofCaspase-3a
6、ndBcl-XLwereobservedbywesternblotandimmunohistochemistrymethods.(DatawereanalysedbystatisticsoftwareofSAS6.12)Result:MTT:thedrug-containedserumofLQHBcouldsignificantlyacceleratecellproliferationandreducetheinhibitionrateofcellgrowthinducedbyH2O2.FCM:theapoptosisratesofNC,M,MS,MLandMHgroupw
7、ere4.85%±0.46%,20.02%±1.37%,21.84%±1.53%,13.56%±0.87%,8.58%±0.74%respectively,andcomparedwithMgroup,theapoptosisrateinMLandMHgroupwasobviouslyreduced.TUNEL:thereweremajorapoptoticcellsinMandMSgroupwhichcellnucleiwerestainedyellow,whileafewwerefoundinMLandMHgro
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