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1、ISSN1007-9319CN14-1218/R华人消化杂志WCJD,1998;6(4):291-295291L-精氨酸在大鼠原位肝移植冷缺血再灌注损伤中的作用121223刁同进姚晓平季兵杨甲梅吴孟超张绍庚EffectsofL-arginineduringischemia-CONCLUSIONL-arginineinastoragesolutiontosupplement-ingNOSPcanprotectthegraftedliverandthelungsfrominjuryreperfusioninjuryinratorthotopicdur
2、ingischemia-reperfusioninorthotopiclivertransplantation.livertransplantationTheNOSPinlivertransplantationmaybeacriticaldeterminantofsuccessfulorgantransplantation,thusprovidingausefulpharma-cologicalapproachtoenhancingliverpreservation.1212DIAOTong-Jin,YAOXiao-Ping,JIBing,YANGJia-Mei,23WUMen
3、g-ChaoandZHANGShao-Geng1DepartmentofGeneralSurgery,ChinesePLA401NavyHospi-tal,Qingdao266071,ShandongProvince,China2EasternHospitalofHepatobiliarySurgery,ChinesePLASecondMilitaryMedicalUniversity,Shanghai200433,China主题词精氨酸/药理学;肝移植;一氧化氮/代谢;再灌注损3伤;大鼠,Wistar;肝/病理学;肺/病理学DepartmentofHepatobiliary
4、Surgery,GeneralHospitalofChi-中国图书资料分类号R6573nesePLA,FuzhouCommandArea,Fuzhou350000,FujianProvince,China摘要Subjectheadingsarginine/pharmacology;liver目的探讨L-精氨酸在大鼠原位肝移植冷缺血再灌注损伤过transplantation;nitricoxide/metabolism;reperfu-程中对移植肝及肺的功能作用.sioninjury;rats,Wistar;liver/pathology;lung/方法应用同基因Wi
5、star大鼠原位肝移植(OLT)动物模型,66pathology只大鼠随机分成3组(n=11):L-精氨酸组(LA)、NOS抑制剂组(L-NAME,LN)及乳酸钠林格液组(LR),供肝保存6h再行原位肝移植术.Abstract结果大鼠原位肝移植缺血再灌注后血清NOx-水平及肝组织AIMToinvestigatethefunctionalofL-arginineingraftedlivercGMP水平迅速下降.应用L-精氨酸以加强一氧化氮生物合成andlungduringischemia-reperfusioninjury(IRI)inratorthotopic途径(NOSP
6、),能显著延长受体的存活率,LR组受体1wk存活livertransplantation(OLT).率为20%,LA组为80%(n=6,P<001vsLR),并能明显改METHODSUsingasyngeneicWistarratorthotopiclivertrans-plantation(OLT)model,66ratswererandomlydividedinto善肝功能;病理检查示L-精氨酸能维持肝细胞完整性,促进肝threegroups(n=11):L-arginine(LA),nitricoxideinhibitor(L-再生,而且对再灌注时合并肺损伤也有明显的
7、保护作用.NOSNAME,LN)andlactatedRinger's(LR)solution.After6hoursof抑制剂则能降低术后受体的存活率,加剧肝功能恶化;病理检preservation,orthotopicimplantationofthedonorliverwasper-查示肝内血栓形成,移植肝及肺组织结构严重破坏及更为严重formed.的炎症反应.RESULTSSerumnitricoxideandcGMPlevelsinthegrafted结论在大鼠原位肝移植缺血再
