糖皮质激素对哮喘大鼠aiolos基因表达与血清ige的影响

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1、遵义医学院硕七学位论文中文摘要关键词：Aiolos基因IgE地塞米松支气管哮喘大鼠遵义医学院硕士学位论文英文摘要曼曼鼍一_一_一．!I．II--．皇曼皇曼皇曼曼曼曼鼍寡量量鼍曼曼量鼍量曼曼曼皇曼量曼曼皇曼曼量笪曼曼曼鼍置曼曼曼曼量皇曼曼曼曼曼曼量量曼量曼TheeffectofglucocorticoidontheAiolosexpressionandthelevelofIgEinasthmaratsAbstractObjective：ToinvestigatetheexpressionofAiolosgeneandirnmu

2、noglobulinI西onasthmaticratandthemechanisimofdexamethasone(DXM)interventionbymeasuringtheexpressionofAiolosmRNAintheperipheralbloodlymphocytesandIgEinthebloodofasthmaticratandthatafterDXMintervention．ToanalyzetherelationshipofAiolosgeneandimmunoglobulinIgEinasthmame

3、chanisiminordertoftu'therexploretheMethods：ThirtySDratswererandomlydividedintothreegroups、析tll10ratsineachgroup：thecontrolgroup，theasthmagroup，andtheDXMgroup．TheasthmaratsmodelwereestablishedbyimmunizedandchallengedwithOVA．Onthe1stdayandthe8thdaytheasthmagroupwereg

4、ivenallintraperitonealinjectionof1ml1％OVAsalineeomplexedwinl10％AL(OH)3．Fromthe15thday．ultrasonicnebulizationwith1％OVAsalinewereusedintheagth／nagroupfor蛐minuteseveryday，itrepeatedfourteendays．TheDXMgroupwereinlraperitonealinjectionDXM1hourbeforeultrasonicnebulizatio

5、neverytime．ThecontrolgroupsensitizedandstimulatedwithsalineinsteadofOVA．24hoursafterthelastchallenge，thevenousbloodandthelungtissuewerecollectedunderanesthesia．TheestablishmentofasthmamodelwasjudgedbythehematoxylinandEosin(HE)stainoflunghistologiesectionofratandthe

6、symptomsofrat．TheexpressionofAiolosgeneWasdetectedbyRT·PCRmethod．IgElevelinperipheralbloodwasdeterminedby趾ELISAtest．Results：Theratmodelofasthmawereestablishedsuccessfully．Compared、)l，inlthecontrolgroup，theasthmagrouphadaobviouslyasthma-likesymptoms：shortnessofbreat

7、h,abdominalcramps，andcyanosis．Andthebronchopulmonaryhistologicobservationsoftheasthmagroupshowedthenumberofeosinophils，lymphoeytes，neu仃ophilsandotherinflammatorycellswereincreasedobviously，whichindicatedthattheratasthmamodelwasestablishedsuccessfully．Comparedwithth

8、easthmagroup，there·3·遵义医学院硕+学位论文英文摘要1=i．III!,III曼曼曼曼鲁量鲁曼皇量皇曼舅篁werelessasthma-likesymptomsintheDXMgroup．Thecontrolgrouphadnoobviousabnormalities．R